In vitro hemolytic activity of Lonomia obliqua caterpillar bristle extract on human and Wistar rat erythrocytes

Toxicon : Official Journal of the International Society on Toxinology
Carla Simone SeibertIda S Sano-Martins

Abstract

Human accidental envenomation caused by skin contact with the bristles of Lonomia obliqua caterpillar causes coagulation and fibrinolysis disorders. Alterations of hematologic parameters are observed only in severe cases of envenomation, but with no clinical evidence of intravascular hemolysis. However, since we have observed intravascular hemolysis in preliminary studies using Wistar rats as an experimental model for investigating L. obliqua envenomation, the objective of the present study was to investigate the in vitro hemolytic activity of the bristle extract of L. obliqua caterpillars on human and rat erythrocytes. Our results showed that the bristle extract has indirect and direct hemolytic activity on human and rat erythrocytes, although direct hemolytic activity was only observed at higher bristle extract concentrations. We also observed that the bristle extract has a proteolytic activity on band 3 of human and rat erythrocyte membranes. Thus, crude L. obliqua bristle extract was found to contain at least two components with hemolytic activity on erythrocytes, a phospholipase enzyme and another protein with a direct activity on the erythrocyte membrane.

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Citations

Mar 23, 2004·Toxicon : Official Journal of the International Society on Toxinology·Lúcia de Castro BastosCarlos Rogério Tonussi
Jan 23, 2013·Cytotechnology·Tiago Elias HeinenAna Beatriz Gorini da Veiga
Feb 16, 2010·Toxicon : Official Journal of the International Society on Toxinology·Carla Simone SeibertIda S Sano-Martins
Jul 16, 2008·Seminars in Nephrology·Regina C R M AbdulkaderEmmanuel A Burdmann
Mar 28, 2008·Toxicon : Official Journal of the International Society on Toxinology·Antônio F M PintoJay W Fox
Feb 27, 2007·Toxicon : Official Journal of the International Society on Toxinology·Linda Christian Carrijo-Carvalho, Ana Marisa Chudzinski-Tavassi
Dec 26, 2006·Toxicon : Official Journal of the International Society on Toxinology·C B BohrerC Termignoni
Oct 27, 2004·Toxicon : Official Journal of the International Society on Toxinology·Carla Simone SeibertIda Sigueko Sano-Martins
Oct 27, 2004·Toxicon : Official Journal of the International Society on Toxinology·Gustavo Henrique da SilvaMaria Alice da Cruz-Höfling
Jul 31, 2004·Toxicon : Official Journal of the International Society on Toxinology·Jairo José Caovilla, Elvino José Guardão Barros
Jul 31, 2004·Toxicon : Official Journal of the International Society on Toxinology·A T RamosO A Sant'Anna
Mar 3, 2006·Biochemical and Biophysical Research Communications·Carla Simone SeibertIda Sigueko Sano-Martins
Dec 20, 2005·Toxicon : Official Journal of the International Society on Toxinology·Antônio F M PintoJorge A Guimarães
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Feb 9, 2012·Toxicon : Official Journal of the International Society on Toxinology·M BarriosB Guerrero
Jun 21, 2016·Toxicon : Official Journal of the International Society on Toxinology·Diva Denelle Spadacci-MorenaJuliana Mozer Sciani
Aug 8, 2015·Revista da Associação Médica Brasileira·Silviane Maggi, Gustavo Adolpho Moreira Faulhaber
Jun 25, 2005·Journal of Applied Physiology·Umit Kemal SentürkOuz K Baskurt
Sep 14, 2004·Revista Da Sociedade Brasileira De Medicina Tropical·Maria Sueli CorrêaSávio Silva Santos

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