PMID: 6967079Jul 1, 1980Paper

In vitro hippocampal uptake of tritiated serotonin (3H-5HT): A morphological, biochemical, and pharmacological approach to specificity

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
E C Azmitia, W F Marovitz

Abstract

The in vitro uptake of tritiated serotonin (3H-5HT) into hippocampal slices was measured in Ringer's solution (37 degrees C) containing pargyline, ascorbic acid, and dextrose. The specific uptake of 3H-5HT rose asymptotically as the 3H-5HT molarity was increased from 5 x 10(-10) to 1.5 x 10(-6) M. Linear regression analysis gave a Km value for the specific uptake of 1.4 x 10(-7) M. The nonspecific binding (NSB) was the amount of 3H-5HT retained by the slices following incubation in a medium with a very large excess of unlabeled 5-HT added to dilute the specific uptake of 3H-5HT. This NSB increased with increasing molarity of 3H-5HT, and was linearly related to 3H-5HT concentrations between 5 x 10(-9) and 1.5 x 10(-6) M. The ratio of specific uptake to NSB was highest at 5 x 10(-8) M (2.75) and lowest at 1.5 x 10(-6) M of 3H-5HT (0.54). Competition studies with noradrenaline, desipramine (a noradrenergic uptake blocker), fluoxetine (a 5-HT uptake blocker), and tryptophan confirmed the specificity of the 3H-5HT uptake mechanism. Radioautographic studies of in vitro incubated hippocampal slices showed silver grain aggregates at 3H-5HT specific uptake sites. Addition of an excess of unlabeled 5-HT to the slices, or the use of hippo...Continue Reading

Citations

Jan 1, 1992·Glia·E J AndersonH K Kimelberg
Jun 7, 1985·European Journal of Pharmacology·F AmentaP Geppetti
Jan 1, 1987·Neuroscience·E C Azmitia, P M Whitaker-Azmitia
Jan 1, 1990·Neuroscience·S Oleskevich, L Descarries
Apr 2, 2004·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Tal BarkanMoshe Rehavi
May 26, 2012·TheScientificWorldJournal·Laura Cristina BerumenGuadalupe García-Alcocer
Sep 18, 2003·Journal of Neuroscience Research·Daniel Reyes-HaroJesús García-Colunga

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