In vitro hydroxyapatite adsorbed salivary proteins

Biochemical and Biophysical Research Communications
Rui VitorinoFrancisco M L Amado

Abstract

In spite of the present knowledge about saliva components and their respective functions, the mechanism(s) of pellicle and dental plaque formation have hitherto remained obscure. This has prompted recent efforts on in vitro studies using hydroxyapatite (HA) as an enamel model. In the present study salivary proteins adsorbed to HA were extracted with TFA and EDTA and resolved by 2D electrophoresis over a pH range between 3 and 10, digested, and then analysed by MALDI-TOF/TOF mass spectrometry and tandem mass spectrometry. Nineteen different proteins were identified using automated MS and MS/MS data acquisition. Among them, cystatins, amylase, carbonic anhydrase, and calgranulin B, were identified.

References

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Jul 1, 1995·Journal of Dental Research·F A ScannapiecoM J Levine
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Mar 30, 2004·Proteomics·Rui VitorinoFrancisco M L Amado

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Citations

Mar 20, 2010·Journal of Materials Science. Materials in Medicine·Guangping XieJie Wei
Jul 12, 2013·PloS One·Young Ho LeeWalter L Siqueira
Apr 25, 2006·European Journal of Oral Sciences·Rui VitorinoFrancisco M L Amado
Sep 26, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Ann R HolmesPatrick Dawes
May 28, 2014·Microbial Pathogenesis·Eric ChabrièreDidier Raoult
Jun 15, 2016·International Journal of Molecular Sciences·Noorjahan Laila HuqEric C Reynolds
Nov 16, 2010·Journal of Dental Research·E E McDonaldW L Siqueira
Dec 25, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tailin GuoJie Weng
May 23, 2007·Biomedical Chromatography : BMC·Rui VitorinoFrancisco M L Amado
Feb 13, 2008·Journal of Separation Science·Rui VitorinoFrancisco M L Amado
Dec 15, 2018·Proteomics. Clinical Applications·Simone TrautmannMatthias Hannig
Apr 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuebo LiuJiyao Li
Nov 28, 2020·Clinical Biochemistry·Mehmet Arif Icer, Hilal Yıldıran
Sep 2, 2006·Journal of Proteome Research·Eric NeyraudEric Dransfield

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