In vitro identification and characterization of an early complex linking HIV-1 genomic RNA recognition and Pr55Gag multimerization

The Journal of Biological Chemistry
Ariel RoldanMark A Wainberg

Abstract

The minimal protein requirements that drive virus-like particle formation of human immunodeficiency virus type 1 (HIV-1) have been established. The C-terminal domain of capsid (CTD-CA) and nucleocapsid (NC) are the most important domains in a so-called minimal Gag protein (mGag). The CTD is essential for Gag oligomerization. NC is known to bind and encapsidate HIV-1 genomic RNA. The spacer peptide, SP1, located between CA and NC is important for the multimerization process, viral maturation and recognition of HIV-1 genomic RNA by NC. In this study, we show that NC in the context of an mGag protein binds HIV-1 genomic RNA with almost 10-fold higher affinity. The protein region encompassing the 11th alpha-helix of CA and the proposed alpha-helix in the CA/SP1 boundary region play important roles in this increased binding capacity. Furthermore, sequences downstream from stem loop 4 of the HIV-1 genomic RNA are also important for this RNA-protein interaction. In gel shift assays using purified mGag and a model RNA spanning the region from +223 to +506 of HIV-1 genomic RNA, we have identified an early complex (EC) formation between 2 proteins and 1 RNA molecule. This EC was not present in experiments performed with a mutant mGag pro...Continue Reading

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Citations

May 17, 2012·Journal of Medicinal Chemistry·Sebastian BreuerBruce E Torbett
Mar 4, 2011·Journal of Virology·Colleen M NovielloEric Barklis
Nov 6, 2007·Journal of Virology·Scott P KenneyLeslie J Parent
Dec 3, 2010·PLoS Pathogens·Sebla B Kutluay, Paul D Bieniasz
Jun 24, 2020·Biophysical Journal·Emmanuel BoutantSerena Bernacchi

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