In vitro imaging of thyroid tissues using two-photon excited fluorescence and second harmonic generation

Photomedicine and Laser Surgery
Zufang HuangChao Li

Abstract

To evaluate the feasibility of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging to discriminate the normal, nodular goiter and papillary cancerous thyroid tissue. In total, 45 fresh thyroid specimens (normal, 15; nodular goiter, 12; and papillary cancerous, 18) from 31 subjects were directly imaged by the TPEF and SHG combination method. The microstructure of follicle and collagen structure in thyroid tissue were clearly identified, morphologic changes between normal, nodular goiter, and papillary cancerous thyroid tissue were well characterized by using two-photon excitation fluorescence. SHG imaging of the collagen matrix also revealed the differences between normal and abnormal. Our preliminary study suggests that the TPEF and SHG combination method might be a useful tool in revealing pathologic changes in thyroid tissue.

References

Mar 14, 2002·Lasers in Surgery and Medicine·Gregory M PalmerNirmala Ramanujam
Oct 7, 2006·American Journal of Respiratory and Critical Care Medicine·Luc ThibervilleGenevieve Bourg Heckly
Jan 11, 2007·Journal of Biomedical Optics·Jonathan M LevittIrene Georgakoudi
Sep 18, 2007·Journal of Biomedical Optics·William C WargerCharles A DiMarzio
Oct 13, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Nathaniel D KirkpatrickUrs Utzinger
Dec 11, 2007·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Jason N RogartMichael H Nathanson
Apr 2, 2008·Medical Engineering & Physics·Te-Hsuen ChenYi-You Huang
Sep 9, 2008·Journal of Biochemistry·Takanori KiharaToshihiko Hayashi
Sep 20, 2008·Photomedicine and Laser Surgery·Sudha D KamathK K Mahato

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Methods Mentioned

BETA
biopsy
fluorescence-imaging

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