PMID: 9419449Jan 7, 1998Paper

In vitro immunization and expansion of antigen-specific cytotoxic T lymphocytes for adoptive immunotherapy using peptide-pulsed dendritic cells

Critical Reviews in Immunology
V TsaiE Celis

Abstract

The design of an effective procedure to sensitize and expand antigen-specific cytotoxic T lymphocytes (CTL) in vitro is essential for the development of effective adoptive cellular immunotherapy protocols for cancer. We have analyzed the capacity of tissue culture-derived dendritic cells (DC) to present specific peptide epitopes to CTL precursors. Our results demonstrate that peptide-pulsed DC were efficient in generating CTL responses specific for various viral and tumor epitopes. Furthermore, IL-7 and IL-10 potentiated the ability of the peptide-pulsed DC to trigger antigen-specific CTL responses. The CTL generated using this procedure efficiently recognized the naturally processed antigens and could be expanded approximately 100- to 1000-fold in tissue culture in 10 to 15 days without a loss of activity and specificity. The results and procedures described herein may facilitate the development of effective CTL-based adoptive immunotherapy for chronic viral diseases and cancer.

Citations

May 14, 2005·Cancer Immunology, Immunotherapy : CII·Keith L KnutsonMary L Disis
Feb 27, 2008·Archives of Dermatological Research·Yan XuHao Cheng
Nov 15, 2000·La Revue de médecine interne·J BartholeynsJ L Romet-Lemonne
Jul 19, 2002·ANZ Journal of Surgery·Veronica YaoJohn C Hall
Jun 17, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David A RodebergEsteban Celis
Nov 3, 2012·PloS One·Adel BenlahrechSteven Patterson
Feb 10, 2006·Cancer Immunology, Immunotherapy : CII·Saskia J A M SantegoetsTanja D de Gruijl
Sep 8, 2004·International Immunology·Anita H UndaleEsteban Celis
Oct 26, 1999·Immunobiology·M Zöller, S Matzku
Feb 2, 2006·International Journal of Cancer. Journal International Du Cancer·David A RodebergEsteban Celis
May 3, 2000·Journal of Hematotherapy & Stem Cell Research·D SkeaD Bell
Jan 5, 2002·Expert Opinion on Biological Therapy·Keith L KnutsonMary L Disis
Jun 15, 2000·Immunological Investigations·H KobayashiE Celis
Mar 28, 2008·Immunological Reviews·Richard Kennedy, Esteban Celis
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J S SerodyJ A Frelinger
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shabnam TangriAlessandro Sette
Sep 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marco W J SchreursErik Hooijberg
Oct 21, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sujatha MuralidharanGianpietro Dotti
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C N BaxevanisM Papamichail
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E KeoghA Sette
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·June Kan-MitchellMichal Bereta
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yongqing LiCassian Yee
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K J MalmbergR Kiessling

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