In vitro immunomodulatory activity of celastrol against influenza A virus infection.

Immunopharmacology and Immunotoxicology
Niloofar KhaliliHedayatollah Shirzad

Abstract

The influenza A virus (IAV) causes severe respiratory disease that remains a leading reason for morbidity and mortality. Previous studies have indicated that influenza complications in addition to viral replication are due to overproduction of pro-inflammatory cytokines. Therefore, a new compound is needed to be used with current antiviral drugs to modulate overproduction of pro-inflammatory cytokines in IAV infection. This study investigated the effect of celastrol on mRNA expression and concentration levels of the pro-inflammatory cytokines tumor necrosis factor alpha (TNFα) and interleukin-6 (IL6) that are induced by influenza A/Puerto Rico/8/34 (H1N1; PR8) in Madin-Darby Canine Kidney (MDCK) cells. The effect of this compound on virus titration and viral mRNA expression was also investigated. Confluent MDCK cells were infected with influenza virus (H1N1; PR8) and treated with celastrol at different concentrations. After incubation, mRNA expression and concentrations of TNFα and IL6 were investigated using real-time polymerase chain reaction (PCR) and ELISA. The viral mRNA expression and virus titration were investigated using real-time PCR and 50% tissue culture infectious dose (TCID50) assay, respectively. mRNA expression ...Continue Reading

References

May 9, 2000·Veterinary Microbiology·K Van Reeth
Feb 13, 2001·The Journal of Clinical Investigation·J HiscottP Génin
Jun 26, 2001·Journal of Medical Virology·L KaiserF G Hayden
Mar 5, 2003·Trends in Molecular Medicine·Stephan LudwigThorsten Wolff
May 30, 2003·The EMBO Journal·M Gabriella SantoroCarla Amici
Sep 1, 2004·Biochemical and Biophysical Research Communications·Guillaume F PinnaChristian D Muller
Dec 17, 2004·Nature·Christina E MillsMarc Lipsitch
Feb 14, 2006·Cellular Microbiology·Stephan LudwigThorsten Wolff
Aug 22, 2006·Mini Reviews in Medicinal Chemistry·Nguyen-Hai Nam
Jan 18, 2008·The New England Journal of Medicine·UNKNOWN Writing Committee of the Second World Health Organization Consultation on Clinical Aspects of Human Infection with Avian InfTimothy M Uyeki
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Margaret A Scull, Charles M Rice
Feb 8, 2011·Microbes and Infection·Sophie A ValkenburgKatherine Kedzierska
Jan 30, 2014·Nutrients·Jong Seok LeeSang-Moo Kang
Apr 26, 2014·Nature Reviews. Immunology·Akiko Iwasaki, Padmini S Pillai
Jul 22, 2014·Trends in Immunology·Nicole L La Gruta, Stephen J Turner
Jul 21, 2015·Cellular & Molecular Immunology·Qiang LiuZhan-qiu Yang
Aug 8, 2015·Antimicrobial Agents and Chemotherapy·Hussein TraboulsiMartin V Richter
Dec 18, 2015·Biochemical and Biophysical Research Communications·Ji Hoon ParkJi-Young Min
Jun 21, 2017·Microbial Pathogenesis·Mohammad-Taghi MoradiFatemeh Fotouhi

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Citations

Apr 30, 2020·Phytotherapy Research : PTR·Solomon HabtemariamSeyed Mohammad Nabavi
Jun 17, 2021·Phytotherapy Research : PTR·Myriam MerarchiManoj Garg

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