Abstract
Evaluation of the in vitro immunomodulatory potential of Artemisia indica Willd. methanolic extract in chicken lymphocyte culture system through lymphocyte (B and T cells) proliferation assay, after standardizing the maximum non-cytotoxic dose (MNCD) in chicken lymphocytes. Fresh aerial parts of A. indica Willd. (family: Asteraceae) specimens were collected (altitude 1560 m), gotten authenticated, processed, dried, and Soxhlet extracted to yield methanolic extract (AME). Chicken splenocytes were isolated from spleens collected from healthy birds; lymphocytes were separated by density gradient centrifugation, percentage cell viability determined and final cell count adjusted to 107 cells/ml in RPMI-1640 medium. MNCD of AME in chicken lymphocytes was determined through 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide dye reduction assay. Immunomodulatory potential of AME was evaluated through lymphocytes proliferation or B and T cells blastogenesis assay in the presence of appropriate mitogens, namely, lipopolysaccharide (LPS) and concanavalin A (Con A), respectively. Maximum concentration of AME exhibiting 100% cell viability (MNCD) was 200 μg/ml and was selected for further in vitro analysis. The in vitro exposure o...Continue Reading
References
Jan 1, 1988·Pharmacology·R KalsiJ P Barthwal
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Feb 1, 1995·International Journal of Immunopharmacology·M IshizukaT Takeuchi
Sep 1, 1993·Journal of Dairy Science·A Bendich
Jun 1, 1997·Journal of Animal Science·D M WebelR W Johnson
Apr 9, 2001·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·E M Anam
Oct 24, 2002·Biological & Pharmaceutical Bulletin·Patricia ValentãoMaria de Lourdes Bastos
Nov 26, 2002·Current Biology : CB·Paul E BoardmanSimon J Hubbard
Mar 12, 2003·Mutation Research·Okezie I Aruoma
Sep 19, 2003·Phytotherapy Research : PTR·Alessandra RussoAngelo Vanella
Oct 24, 1964·Nature·E KLEIN, G KLEIN
May 26, 2004·Fitoterapia·Y M FanZ X Zhang
Jul 6, 2004·International Immunopharmacology·Xiangfeng KongXiangrui Li
Jan 24, 2006·International Immunopharmacology·Igor A Schepetkin, Mark T Quinn
Mar 4, 2008·Cellular Immunology·Cheng-Rui LiDavid D Lo
Mar 8, 2008·Phytochemistry·Gang XieMark T Quinn
Mar 11, 2008·International Immunopharmacology·Jin-Ah LeeJoo-Hung Park
Oct 1, 2009·International Immunopharmacology·Chan-Ho LeeSun-Mee Lee
Oct 26, 2010·Oxidative Medicine and Cellular Longevity·Jaouad Bouayed, Torsten Bohn
Dec 20, 2011·Journal of Ethnopharmacology·Lívia de Paulo PereiraMaria Gonçalves Pereira
Apr 20, 2013·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Eunjeong ChoiGunhee Kim
Aug 31, 2013·Carbohydrate Polymers·Xiaoli BaoMinbo Lan
Nov 14, 2013·ISRN Inflammation·Khan MubashirAkbar Masood
Jan 31, 2014·Chemistry Central Journal·Diana ObistioiuViorica Chiurciu
Feb 11, 2014·Carbohydrate Polymers·Marília Locatelli Corrêa-FerreiraCarmen Lúcia Oliveira Petkowicz
Jan 22, 2015·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Wan-Su KimWonyong Kim
May 12, 2015·Mediators of Inflammation·Chenchen ShiLifei Hou
May 12, 2015·Journal of Immunology Research·Rodrigo ArreolaDaniel Ortuño-Sahagún
Citations
Mar 4, 2020·Nutrients·Yue ZhaoMeiyu Xu
Dec 6, 2018·Nutrients·Hong LiuMeiyu Xu
Jan 14, 2021·Cephalalgia : an International Journal of Headache·Elise Øien SørnesMattias Linde
Mar 2, 2021·Veterinary World·Ekaterina Romanovna VasilevskayaAngelina Igorevna Fokina