PMID: 1194352Nov 1, 1975Paper

In vitro incorporation of (3H)threonine and (3H)glucose by the mucous and serous cells of the human bronchial submucosal gland. A quantitative electron microscope study

The Journal of Cell Biology
B Meyrick, L Reid

Abstract

Incorporation of [3H]threonine and [3H]glucose by the mucous and serous cells of the human bronchial submucosal gland has been studied over 8 h using, for the first time in vitro pulse labeling and electron microscope autoradiography. In assessing the autoradiographs, two methods were compared, the circle analysis and the recently described hypothetical grain analysis. Preliminary studies showed formaldehyde to be the most suitable fixative. Chemical analysis of tissue revealed that [3H]threonine was incorporated into the polypeptide moiety of the bronchial gland product and that metabolites of [3H]-glucose were incorporated into the carbohydrate. Tritiated threonine was first localized in the endoplasmic reticulum of both mucous and serous cells and later migrated to the Golgi apparatus, while metabolites of [3H]glucose localized first mainly in the Golgi apparatus. From here, both radioactive precursors were next identified in vacuoles and, finally, in secretory granules. The mucous cell incorporated strikingly more of both radioactive precursors than the serous cell. Thus, it seems that oligosaccharides of mucous and serous cell glycoproteins are synthesized mainly in the Golgi apparatus and added there to the polypeptide co...Continue Reading

References

Feb 1, 1975·Journal of Clinical Pathology·D Y Mason, C R Taylor
Apr 1, 1967·The Journal of Cell Biology·T Peters, C A Ashley
Apr 1, 1973·The Journal of Cell Biology·D M Parry, N M Blackett
May 1, 1972·The Journal of Cell Biology·J D CastleG E Palade
Jun 1, 1972·Experimental and Molecular Pathology·J Sturgess, L Reid
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Aug 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T Vanha-Perttula, P M Grimley
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Dec 1, 1967·The Journal of Cell Biology·S Hodson, J Marshall
Jan 1, 1959·Experimental Cell Research·O A TROWELL

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Citations

May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Apr 1, 1976·Postgraduate Medical Journal·L Reid
Dec 1, 1980·The Journal of Clinical Investigation·J H ShelhamerM Kaliner
Jan 1, 1984·Experimental Lung Research·J H ShelhamerM Kaliner
Feb 1, 1983·Experimental Lung Research·L ReidS Coles
Dec 1, 1978·Experimental and Molecular Pathology·S J Coles, L Reid
Oct 1, 1975·Postgraduate Medical Journal·L Reid
Aug 1, 1984·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·C A PatowM Kaliner
Dec 1, 1987·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Susan J Kimber, Peter R Bagley

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