PMID: 58941Jan 1, 1976

In vitro induction and measurement of hemolytic plaque forming cells in man

Journal of Immunological Methods
H M Dosch, E W Gelfand

Abstract

Following co-cultivation with sheep red cells or ovalbumin, Hypaque-Ficoll-separated human tonsillar lymphocytes were demonstrated to generate specific hemolytic PFC with maximum numbers at day 5-7. PFC were enumerated on poly-L-lysine coupled red cell monolayers in Microtest-II-plates. Plaque formation appeared to be puromycin-sensitive, complement-dependent and showed clear specificity for the antigen present during the inductive culture. Treatment of PFC with mu-chain specific antisera and complement resulted in complete inactivation of PFC; gamma-chain antisera had no effect. The development of such a simple and sensitive assay system permits the analysis of cellular interactions required for the induction of PFC responses in man.

References

Apr 26, 1963·Science·N K Jerne, A A Nordin

Citations

Sep 30, 1982·Journal of Immunological Methods·T EgelandO J Mellbye
Dec 16, 1983·Journal of Immunological Methods·P R MacdonaldP S Crosier
Jan 1, 1983·International Journal of Immunopharmacology·E J PiskoR A Turner
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D J VolkmanA S Fauci
Nov 1, 1990·Scandinavian Journal of Immunology·P A MarwitzB J Zegers
Jan 1, 1979·Immunological Reviews·R E BallieuxB J Zegers
Jan 1, 1979·Immunological Reviews·R S Geha
Jun 1, 1983·Annals of the Rheumatic Diseases·R A TurnerW J Treadway
Apr 1, 1978·The Journal of Clinical Investigation·E W GelfandI H Fox
Dec 1, 1978·The Journal of Clinical Investigation·R H Stevens, A Saxon
Jun 1, 1981·Clinical Immunology and Immunopathology·H C RümkeW P Zeijlemaker
Jan 1, 1982·Annals of the New York Academy of Sciences·M D Benson, M A Aldo-Benson
Sep 1, 1982·Arthritis and Rheumatism·E J PiskoS L Foster
Nov 1, 1980·Arthritis and Rheumatism·E C KeystoneL Poplonski

Related Concepts

Anti-Antibodies
Antibody-Secreting Cells
Antigenic Specificity
Hemolytic Complement
Hemolytic Plaque Technique
Phytomitogens
Puromycin Dihydrochloride

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