In vitro inhibition of human glioblastoma cell line invasiveness by antisense uPA receptor

Oncogene
S MohanamJ S Rao

Abstract

The cell surface urokinase-type plasminogen activator receptor (uPAR) has been shown to be a key molecule in regulating plasminogen-mediated extracellular proteolysis. To investigate the role of uPAR in invasion of brain tumors, human glioblastoma cell line SNB19 was stably transfected with a vector capable of expressing an antisense transcript complementary to the 300 base pair of the 5' end of the uPAR mRNA. Parental and stably transfected (vector, sense, and antisense) cell lines were analysed for uPAR mRNA transcript by Northern blot analysis, and receptor protein levels were measured by radioreceptor assays and Western blotting. Significant reduction of uPAR sites was observed in the antisense transfected cell lines. The levels of uPAR mRNA were significantly decreased in antisense clones compared to control, vector and sense clones. The invasive potential of the cell lines in vitro was measured by Matrigel invasion assay and migration of cells from spheroids to monolayers. The antisense transfected cells showed a markedly lower level of invasion and migration than the controls. The antisense clones were more adhesive to the ECM components compared to parental, vector and sense clones. All transfected (vector, sense and an...Continue Reading

Citations

Jun 19, 2001·International Journal of Cancer. Journal International Du Cancer·V EllenriederT M Gress
May 2, 2006·Clinical & Experimental Metastasis·Crispin R DassPeter F M Choong
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Anita C BellailErwin G Van Meir
Nov 26, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S K ChintalaJ S Rao
Sep 9, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M R Del BigioM Altumbabic
Sep 19, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T Meyer, I R Hart
Mar 15, 2003·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Dina Tsatas, Andrew H Kaye
Oct 18, 2000·Critical Reviews in Oncology/hematology·W Mueller-Klieser
Oct 18, 2000·Critical Reviews in Oncology/hematology·G Fracasso, M Colombatti
Jul 2, 2003·Nature Reviews. Cancer·Jasti S Rao
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jingjing HuSteven L Gonias
Mar 29, 2001·Journal of Hematotherapy & Stem Cell Research·F TögelW Krüger
Jul 20, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christopher S GondiJasti S Rao
Nov 12, 2009·Future Oncology·Ahmed H MekkawyMohammad H Pourgholami
Nov 26, 2013·Molecular Neurobiology·Eli T SayeghAndrew T Parsa
Jul 31, 2013·Biochimica Et Biophysica Acta·Anne Vehlow, Nils Cordes
Jul 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bo HuShi-Yuan Cheng
Nov 5, 2003·Medicinal Research Reviews·Charles Edo de Bock, Yao Wang
Sep 3, 2010·Contrast Media & Molecular Imaging·S Del VecchioM Salvatore
Jan 5, 2002·Hematology/oncology Clinics of North America·S A Rempel
Aug 31, 2010·Molecular and Cellular Neurosciences·Beatriz Valle-ArgosManuel Nieto-Sampedro
Feb 21, 2007·Journal of Pharmacological Sciences·Unmesh JadhavSanjeeva Mohanam
Apr 18, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Heike Allgayer
Jan 17, 2015·Journal of Biotechnology·Delyan P IvanovMartin C Garnett
May 17, 2005·Applied Immunohistochemistry & Molecular Morphology : AIMM·Mohammad SalajeghehThomas W Smith
Oct 11, 2011·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Duoguang WuMinghui Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.