Oct 1, 1983

In vitro insulin resistance of human adipocytes isolated from subjects with noninsulin-dependent diabetes mellitus

The Journal of Clinical Investigation
A KashiwagiJ E Foley

Abstract

To assess possible cellular mechanisms of in vitro resistance in noninsulin-dependent diabetes mellitus (NIDDM), maximum insulin-stimulated glucose transport and utilization and insulin binding were measured in adipocytes isolated from weight-matched normal glycemic subjects and patients with NIDDM. Glucose transport rate was determined by measuring the amount of [U-14C]-D-glucose taken up by incubating adipocytes at trace concentrations of glucose (300 nM), and glucose metabolism by estimating the amount of lactate, CO2, triglyceride, and total glucose carbons retained in the cells following incubating at 5.5 mM glucose. Insulin binding was measured at 50, 100, and 200 pM [mono125I-tyrosinyl A14]insulin. Both maximum insulin-stimulated glucose transport and utilization in adipocytes from diabetic subjects were 40% (P less than 0.01) and 32% (P less than 0.05) lower, respectively, than values obtained for subjects with normal glucose tolerance. In addition, the maximum capacity of glucose transport was correlated with the maximum capacity of glucose utilization (r = 0.81, P less than 0.001). Furthermore, fasting plasma glucose concentrations of diabetic subjects were negatively correlated with both maximum insulin-stimulated gl...Continue Reading

  • References23
  • Citations104

Mentioned in this Paper

Metabolic Process, Cellular
Diabetes Mellitus, Non-Insulin-Dependent
Insulin Sensitivity
Fat Pad
Lactate
Glucose, (beta-D)-Isomer
Insulin Resistance
Plasma Fasting Glucose Measurement
Lactic Acid Measurement
Lactates

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