In vitro interaction of viral haemorrhagic septicaemia virus and leukocytes from trout (Oncorhynchus mykiss) and turbot (Scophthalmus maximus)

Veterinary Immunology and Immunopathology
C TafallaBeatriz Novoa

Abstract

Viral haemorrhagic septicaemia virus (VHSV), a well known salmonids pathogen, has also been reported to be pathogenic for turbot (Scophthalmus maximus). In the present work, the replication of VHSV was studied in vitro in turbot head kidney macrophages and blood leukocytes. VHSV was able to infect both primary cultures and viral titer increased with time, either inside the cells or in the supernatant. However, no cytopathic effect was observed during the experiments and the titers were always lower than those obtained in the fish cell lines. The number of trout and turbot macrophages after several days of in vitro infection with VHSV was compared with uninfected controls by viable cell count but no significant differences were observed. The number of cells supporting viral replication evaluated by immunofluorescence in trout and turbot was low (8 and 1.7%, respectively). Respiratory burst activity of head kidney macrophages was assayed at different days post-infection, but no significant differences were found between the control and the infected cultures neither in trout nor turbot.

Citations

Jan 11, 2000·Veterinary Immunology and Immunopathology·C TafallaB Novoa
Jun 5, 2007·Molecular Immunology·L Poisa-BeiroB Novoa
Jun 16, 2016·Frontiers in Physiology·Patricia PereiroBeatriz Novoa
Nov 23, 2017·Frontiers in Immunology·Patricia PereiroBeatriz Novoa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.