In vitro interference with hepatocellular transport of taurocholate by 1,1,2-trichloro-1,2,2-trifluoroethane

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M Neghab, N H Stacey

Abstract

In recent years workers in our laboratory have shown that several industrial chlorinated aliphatic hydrocarbon solvents interfere with the transport of bile acids by hepatocytes and this interference may account for the raised concentration of serum bile acids that has been observed after occupational exposure to solvents. There has been concern about the effects on workers of a selective solvent used in degreasing electrical equipment, 1,1,2-trichloro-1,2,2-trifluoroethane (FC 113). However, this compound has not been investigated for effects on bile acid transport. Therefore we undertook the present study to examine the direct in vitro effects of FC 113 on uptake and efflux of bile acids by isolated rat hepatocytes. FC 113, at non-cytotoxic doses after a 20-min equilibration time, showed significant (P < 0.05) inhibitory effects on the initial rate of uptake of taurocholate (TC), whereas accumulation of TC over an extended incubation time was not affected. Kinetic analysis revealed a non-competitive inhibition of TC uptake as evidenced by a decline in V(max) and an unaltered K(m). The initial rate of efflux of TC and the continuous efflux of this model substrate from preloaded cells incubated with different doses of solvent w...Continue Reading

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Citations

Dec 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M NeghabN H Stacey
Jan 1, 2000·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals· Masoud Neghab Neill H Stacey
Oct 8, 1997·Journal of Toxicology and Environmental Health·M Neghab, N H Stacey

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