PMID: 2121738Jan 1, 1990Paper

In vitro investigations on induction and reversal of cisplatin resistance in a rat ovarian tumor cell line

Journal of Cancer Research and Clinical Oncology
G Chen, W J Zeller

Abstract

Resistance of a rat ovarian tumor cell line (O-342/DDP) to cisplatin was induced in vitro by stepwise increase of cisplatin concentrations. Both chemosensitive parental cells (O-342) and resistant O-342/DDP cells grow in a monolayer and enter log-phase growth about 24 h after seeding (cell population doubling time in log-phase growth is about 24 h). O-342/DDP cells show a 33-fold resistance to cisplatin as compared to O-342 cells (ID50 = 33 microM in O-342/DDP vs 1 microM in O-342 cells). The intracellular total glutathione (GSH + GSSG) of O-342/DDP cells was twice as high as that of O-342 cells (3.04 vs 1.37 nmol/10(6) cells), while the intracellular GSSG was increased by 26% in O-342/DDP cells compared to O-342 cells. DNA interstrand crosslinks were found to be 8.5 times higher in O-342 cells than in O-342/DDP cells (204 vs 24 rad equiv.), following cisplatin treatment. DNA single-strand breaks were approximately doubled in the sensitive line as compared to the resistant line following exposure to cisplatin. Chromosome analysis uncovered a change in the karyotype of O-342/DDP cell as compared to O-342 cells. In the sensitive line hyperploid (3n) clones, in the resistant line near-diploid clones predominated. Both DL-buthionin...Continue Reading

References

Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·W J ZellerM Kaufmann
Jun 1, 1985·Analytical Biochemistry·W SterzelG Eisenbrand
May 1, 1988·European Journal of Cancer & Clinical Oncology·S SekiyaH Takamizawa

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Citations

Jan 1, 1993·Cancer Chemotherapy and Pharmacology·G Chen, W J Zeller
Jan 1, 1996·Journal of Cancer Research and Clinical Oncology·F SeifertW J Zeller
Jun 19, 2001·Mutation Research·M Kartalou, J M Essigmann
May 8, 1998·Obstetrics and Gynecology·G Coukos, S C Rubin
Apr 13, 2012·American Journal of Clinical Oncology·Kathryn A MasonLuka Milas
May 23, 1998·Experimental Cell Research·Z S ChenS Akiyama

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