PMID: 8585096Nov 1, 1995Paper

In vitro labeling of gonadal steroid hormone receptors in brain tissue sections

Steroids
T J BrownN J MacLusky

Abstract

Autoradiographic methods have been developed for measurement of gonadal steroid receptors in situ in brain tissue sections. Based on principles established previously for estrogen receptors in the rat brain using a 125I-labeled ligand, procedures have been developed for in vitro labeling of estrogen, androgen, and progestin receptors with commercially available tritiated ligands. Addition of protamine sulfate to the incubation buffer precipitates the receptors in situ in the tissue sections, allowing them to be detected autoradiographically after incubation with labeled steroid and subsequent washing to remove unbound and nonspecifically bound ligand. Occupied and unoccupied estrogen receptors can be measured selectively using appropriately modified incubation conditions. In the case of androgen and progestin receptors, unoccupied receptors are readily detected by in vitro labeling of tissue sections, but occupied receptors do not appear to label efficiently. Preliminary data suggest that these methods should be equally applicable to a variety of laboratory animals, including the rat, mouse, guinea pig, and monkey.

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Citations

Dec 5, 1997·The Journal of Comparative Neurology·P J ShughrueI Merchenthaler
Jul 1, 1996·Brain Research. Molecular Brain Research·R J HandaG Hejna
Jan 13, 2012·The Journal of Nutrition, Health & Aging·M MaggioG P Ceda
Oct 18, 2005·The Journal of Comparative Neurology·Betty LorenzLydia L DonCarlos
Mar 13, 2008·The European Journal of Neuroscience·Jian-Min ZhangMargaret M McCarthy
May 15, 2012·The Journal of Comparative Neurology·Ryan T JohnsonCynthia L Jordan
Nov 29, 2005·Neuroscience·L L DonCarlosL-M Garcia-Segura
Nov 3, 2005·Hormones and Behavior·Noah J SandstromMolly A Wasserman
Nov 4, 1998·Frontiers in Neuroendocrinology·G G KuiperJ A Gustafsson
Sep 5, 2002·The Journal of Comparative Neurology·Daniel García-OvejeroLydia L Doncarlos
Oct 17, 2016·Psychoneuroendocrinology·Rene SeigerRupert Lanzenberger

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