In vitro large scale production of megakaryocytes to functional platelets from human hematopoietic stem cells.

Biochemical and Biophysical Research Communications
Pasupuleti Santhosh KumarPotukuchi Venkata Gurunadha Krishna Sarma

Abstract

Megakaryocytopoiesis results in the formation of platelets, which are essential for hemostasis. Decreased production or increased destruction of platelets can cause thrombocytopenia, in which platelet transfusion is the mode of treatment. The present study is aimed in generation of megakaryocytes (MKs) and platelet from human hematopoietic stem cells (HSCs). The purity of HSCs was assessed through Flow cytometry and immunocytochemistry (ICC) studies. These pure HSCs were induced with thrombopoietin (TPO), similarly with Andrographis paniculata extract (APE) for 21 days to generate MKs. The APE is mainly composed of andrographolide which stimulates TPO from the liver, and this binds to CD110 present on the surface of HSCs and triggers the proliferation of HSCs and initiate higher MKs population subsequently, a large number of platelets. The results of the present study showed increased proliferation of HSCs grown in the presence of APE and revealed a high population of CD41a and CD42b positive MKs as enumerated by Flow cytometry compared with TPO induced MKs. These results also concurred with qRT-PCR and western blot analysis. The scanning electron microscopy (SEM) revealed the morphology of differentiated MKs and platelets were...Continue Reading

Citations

Feb 23, 2019·Scientific Reports·Chodimella ChandrasekharPotukuchi Venkata Gurunadha Krishna Sarma
Jul 10, 2020·International Journal of Molecular Sciences·Alessandro AllegraSebastiano Gangemi
May 31, 2019·Emerging Topics in Life Sciences·Moyra LawrenceCedric Ghevaert

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