In vitro lens capsule model for investigation of posterior capsule opacification

Journal of Cataract and Refractive Surgery
Georgia ClearyJohn Marshall

Abstract

A modified dissection technique of donor eyes for investigating posterior capsule opacification (PCO) that preserves normal capsule and zonule architecture is described. The intact crystalline lens-zonule-ciliary body complex is dissected from the globe in one piece and pinned with 8 entomological pins through the ciliary body to a soft silicone ring with an internal diameter of 12.7 mm. The specimen is iridectomized and cataract extraction performed; the specimen is then placed in culture. The capsule is supported by the native zonules and suspended freely within culture medium. The entire capsular bag is visible, allowing observation of lens epithelial cell (LEC) growth. In 15 eyes of 13 donors, the mean time to LEC confluence was 10.4 days +/- 1.4 [SD]. This technique builds on previous capsular bag models, providing a more physiological model for observation of PCO in vitro. No author has a financial or proprietary interest in any material or method mentioned.

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Citations

May 6, 2014·Cell and Tissue Research·Jong Hwa JunJae-Young Kim
Jan 28, 2014·Journal of Cataract and Refractive Surgery·David J SpaltonI Michael Wormstone
May 6, 2015·Experimental Eye Research·Ian Michael Wormstone, Julie Ann Eldred
Jan 11, 2019·Investigative Ophthalmology & Visual Science·Julie A EldredI Michael Wormstone
Apr 22, 2011·Journal of the Royal Society, Interface·Dmitry M GakamskyS Desmond Smith
Mar 1, 2020·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Kanjana OngkasinElisabeth Badens
Sep 26, 2020·Progress in Retinal and Eye Research·I M WormstoneJ A Eldred

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