In vitro lifespan and senescence mechanisms of human nucleus pulposus chondrocytes

The Spine Journal : Official Journal of the North American Spine Society
Seo-Won JeongKi-Won Kim

Abstract

Our previous in vivo study demonstrated that human nucleus pulposus chondrocytes (NPCs) in aging discs exhibited characteristic senescent features such as an increased senescence-associated β-galactosidase (SA-β-gal) expression, shortened telomere, and decreased telomerase activity. The replicative p53-p21-pRB pathway, rather than the stress-induced p16-pRB pathway, played a more important role in the senescence of NPCs in an in vivo condition, although there is a situation in which both the pathways can be activated simultaneously. However, the in vitro lifespan and senescence mechanisms of human NPCs remain unclear. To evaluate the underlying mechanisms of in vitro lifespan and in vitro senescence of the human NPCs and to verify whether the in vitro senescence mechanisms of the human NPC can represent the in vivo aging mechanisms of the cell. An in vitro study. We serially cultivated human NPCs from patients of different ages (35, 42, 55, 66, and 76 years) until the cells reached the end of their in vitro lifespan. During each subcultivation, we calculated NPCs cumulative population doubling level (PDL) and examined senescence markers (SA-β-gal, telomere length, telomerase activity, and p53, p21, pRB, and p16 expressions). Th...Continue Reading

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Citations

Apr 2, 2016·Regenerative Medicine·Michael D WestHal Sternberg
Apr 28, 2016·Oxidative Medicine and Cellular Longevity·Olga KrupkovaKarin Wuertz-Kozak
Sep 19, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Sajjad AshrafRita Kandel
Feb 11, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Ki-Won KimJun-Seok Lee
Jun 11, 2019·Molecular Medicine Reports·Lei DingGuoxiong Xu
Jun 25, 2017·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Sotiris-Spyros VamvakasDimitris Kletsas
Nov 27, 2018·Current Molecular Biology Reports·Prashanti PatilNam Vo
Jun 16, 2019·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Kalliopi AlpantakiAlexander Hadjipavlou
Sep 17, 2020·Oxidative Medicine and Cellular Longevity·Yuang ZhangQixin Chen

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