In Vitro MCF-7 Cells Apoptosis Analysis of Carboplatin Loaded Silk Fibroin Particles

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Nanyak GalamTerin Adali

Abstract

Breast cancer ranks as the fifth leading cause of death worldwide. Chemotherapy is commonly used directly or as neo-adjuvant therapy for the management of breast cancer with its attendant adverse effects, underscoring the need to develop biocompatible bioactive compounds for pharmacological applications. The aim of this study is to encapsulate carboplatin (CP) with silk fibroin protein (SF) by using an ionic gelation method as a drug carrier system and assess the apoptotic effect on MCF-7 breast cancer cells during in vitro studies. The characterization of silk fibroin encapsulated carboplatin (SFCP) microparticles was analyzed by FTIR spectrophotometer, SEM, Mastersizer, and biodegradation methods. The encapsulation efficiency and release profile of SFCP microparticles were analyzed by an indirect UV-Vis spectrophotometric method. An apoptotic screening of MCF-7 cells was carried out with 10-200 µg/mL CP loaded SFCP, which were cultured for 24, 48, and 72 h. Data were analyzed using the Student's t test and analysis of variance. FTIR and drug release studies confirmed an interaction of silk fibroin with the carboplatin moiety. SFCP showed successful encapsulation of the carboplatin moiety. Apoptotic screening showed a dose dep...Continue Reading

References

Oct 1, 1991·Clinical Pharmacokinetics·W J van der Vijgh
Feb 3, 2009·Experimental and Molecular Pathology·Rajesh Singh, James W Lillard
Mar 25, 2009·Nature Reviews. Cancer·Don X NguyenJoan Massagué
Jul 29, 2010·Nanomedicine·Anshu B Mathur, Vishal Gupta
Dec 19, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Bano SubiaSubhas C Kundu
Mar 24, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fatemeh MottaghitalabHossein Hosseinkhani
Mar 24, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·A Abel Lozano-PérezJosé Ruiz
May 6, 2015·Journal of Agricultural and Food Chemistry·José A Tapia-HernándezFrancisco Rodríguez-Félix
Sep 26, 2015·SpringerPlus·Marina A SenchukovaAlexander A Stadnikov
Jan 31, 2016·International Journal of Biological Macromolecules·Terin Adalı, Murat Uncu
Apr 24, 2016·Critical Reviews in Oncology/hematology·Gwo Yaw HoJermaine I G Coward
Jul 12, 2016·Current Drug Metabolism·Huile Gao
Jan 14, 2017·International Journal of Biological Macromolecules·Md Asad KhanM Moshahid A Rizvi
Apr 25, 2017·Journal of Experimental Pharmacology·Abir Kumar PandaGaurisankar Sa
Sep 9, 2017·International Journal of Nanomedicine·Santosh Kumar SinghRajesh Singh
Jul 30, 2018·Critical Reviews in Eukaryotic Gene Expression·Pinar TulayTerin Adali
Nov 30, 2018·International Journal of Biological Macromolecules·Terin AdaliLeila Karimizarandi
Jan 23, 2019·JAMA : the Journal of the American Medical Association·Adrienne G Waks, Eric P Winer
Jun 23, 2019·Colloids and Surfaces. B, Biointerfaces·Duy Toan PhamWaree Tiyaboonchai
Sep 29, 2019·Pharmaceutics·Mhd Anas TomehXiubo Zhao
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohammed Al-ZharaniMuhammad A Wadaan
Nov 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang GuanDong-Feng Pan
Nov 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qi-Yao WeiAndy T Y Lau

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
Infrared Spectroscopy

Software Mentioned

stat
Matersizer
Mastersizer
graphpad prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis