PMID: 11332050May 3, 2001Paper

In vitro measurement of nitazoxanide sensitivity of 4 Giardia duodenalis isolates obtained from different hosts

Revista de investigación clínica; organo del Hospital de Enfermedades de la Nutrición
Martha Ponce-MacotelaMario Noé Martínez-Gordillo

Abstract

In Mexico giardiosis is the most prevalent parasitic disease in children. Treatment with antiparasitics derived from: nitroimidazoles, benzimidazoles and nitrofuranes have been used; but, some of them have undesirable side effects. Recently nitazoxanide (Ntz) was introduced in Mexico; however, there are few studies on Giardia duodenalis susceptibility to Ntz. To determine G. duodenalis sensitivity to Ntz and compare it to tinidazole (Tnz). Assays were performed in four G. duodenalis isolates: INP231087MM, INP210897-AXA1 obtained from humans infections, INP170693HG8, INP300693-CP5 from a cat and a dog, respectively. Half million trophozoites were exposed to different Ntz or Tnz concentrations in TYI-S-33, for 24 hours at 37 degrees C. Trophozoite viability was tested by the colorimetric method of MTT-tetrazolium salts reduction to MTT-formazan. To analyze ultrastructural damage, control and experimental samples were processed by standard electron microscopy methods. Experiments were carried out, in a double blind in triplicate and repeated four times. Results were analyzed by variance analysis. Susceptibility at 100% were in a Ntz concentration ranging from 1 microgram to 7 micrograms and in a Tnz concentration ranging from 1 mi...Continue Reading

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