In vitro melanogenesis inhibition by fluphenazine and prochlorperazine in normal human melanocytes lightly pigmented

Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences
Michał OtrębaEwa Buszman

Abstract

Fluphenazine and prochlorperazine as phenothiazine-class antipsychotic drugs are widely used to treat schizophrenia, however their use is associated with significant side effects such as extrapyramidal symptoms, as well as ocular and skin disorders. Our goal was to determine the effect of fluphenazine and prochlorperazine on cell viability and melanogenesis in lightly pigmented normal human melanocytes. The viability of melanocytes was evaluated by the WST-1 colorimetric assay, while melanin content and tyrosinase activity were tested spectrophotometrically. It has been shown that both phenothiazines induce the concentration-dependent loss in cell viability. The EC50 values were calculated to be 6.13 and 0.63 μM for fluphenazine and prochlorperazine, respectively. Fluphenazine in the concentration of 5.0 μM and prochlorperazine in concentrations of 0.5 and 0.75 μM decreased melanin content and tyrosinase activity. The observed inhibition of melanogenesis may be explained by the decrease of enzyme activity. The demonstrated changes in melanization process in lightly pigmented cells exposed to fluphenazine and prochlorperazine in vitro suggest a significant role of melanin and melanocytes in the mechanisms of undesirable side eff...Continue Reading

References

Oct 24, 2001·Forensic Science International : Synergy·C L WinekT W Balzer
May 14, 2004·Pigment Cell Research·Raymond E Boissy, Prashiela Manga
Oct 4, 2008·Revista brasileira de psiquiatria : orgão oficial da Associação Brasileira de Psiquiatria, Asociación Psiquiátrica de la América Latina·Valéria Barreto Novais e SouzaKarla Feitosa Ximenes Vasconcelos
Dec 3, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Michał OtrebaEwa Buszman
May 10, 2015·British Medical Bulletin·John Lally, James H MacCabe
Jun 25, 2015·Naunyn-Schmiedeberg's Archives of Pharmacology·Michał OtrębaEwa Buszman
Jul 16, 2015·Pigment Cell & Melanoma Research·Marco d'IschiaShosuke Ito
Aug 5, 2015·PLoS Medicine·Joanna Moncrieff

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Citations

Aug 28, 2020·Journal of Applied Toxicology : JAT·Michał Otręba, Leon Kośmider
May 16, 2020·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Bo LiYiling Yu

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