In Vitro Metabolic Transformation of Pharmaceuticals by Hepatic S9 Fractions from Common Carp (Cyprinus carpio).

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Viktoriia BurkinaVladimir Zlabek

Abstract

Water from wastewater treatment plants contains concentrations of pharmaceutically active compounds as high as micrograms per liter, which can adversely affect fish health and behavior, and contaminate the food chain. Here, we tested the ability of the common carp hepatic S9 fraction to produce the main metabolites from citalopram, metoprolol, sertraline, and venlafaxine. Metabolism in fish S9 fractions was compared to that in sheep. The metabolism of citalopram was further studied in fish. Our results suggest a large difference in the rate of metabolites formation between fish and sheep. Fish hepatic S9 fractions do not show an ability to form metabolites from venlafaxine, which was also the case for sheep. Citalopram, metoprolol, and sertraline were metabolized by both fish and sheep S9. Citalopram showed concentration-dependent N-desmethylcitalopram formation with Vmax = 1781 pmol/min/mg and Km = 29.7 μM. The presence of ellipticine, a specific CYP1A inhibitor, in the incubations reduced the formation of N-desmethylcitalopram by 30-100% depending on the applied concentration. These findings suggest that CYP1A is the major enzyme contributing to the formation of N-desmethylcitalopram. In summary, the results from the present ...Continue Reading

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Oct 1, 1995·The Journal of Clinical Investigation·T A HazinskiA DeMatteo
Dec 9, 2003·Research in Veterinary Science·B SzotákováL Skálová
Jun 5, 2004·Marine Environmental Research·Eun-Young KimShinsuke Tanabe
Nov 18, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·R Scott ObachLarry M Tremaine
Feb 22, 2005·Environmental Toxicology and Chemistry·Bryan W BrooksRussell J Lewis
Sep 13, 2011·Journal of Veterinary Pharmacology and Therapeutics·M L MatéG Virkel
Mar 17, 2012·Journal of Psychosocial Nursing and Mental Health Services·Robert H Howland
Nov 28, 2012·Analytical and Bioanalytical Chemistry·Lucie StuchlíkováLenka Skálová
Apr 23, 2013·Environmental Toxicology and Chemistry·Kristin A ConnorsBryan W Brooks
May 15, 2015·Clinical Pharmacology and Therapeutics·J K HicksUNKNOWN Clinical Pharmacogenetics Implementation Consortium
Aug 19, 2015·Environmental Toxicology and Pharmacology·Viktoriia BurkinaGalia Zamaratskaia
Feb 24, 2016·Drug Metabolism Letters·Samantha J RichardsonMehran F Moghaddam
Apr 12, 2016·Rapid Communications in Mass Spectrometry : RCM·Olga KobaRoman Grabic
Nov 26, 2016·Toxicology·Viktoriia BurkinaGalia Zamaratskaia
May 2, 2018·The Science of the Total Environment·Sidika SakalliVladimir Zlabek
May 19, 2018·Journal of the American College of Cardiology·Ileana L PiñaHector O Ventura
Aug 5, 2018·Environmental Toxicology and Pharmacology·Viktoriia BurkinaGalia Zamaratskaia
Jun 30, 2019·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Sherry N N DuGraham R Scott

❮ Previous
Next ❯

Citations

Jul 2, 2021·Analytical and Bioanalytical Chemistry·Noemí Molina-FernándezJon Sanz-Landaluze

❮ Previous
Next ❯

Software Mentioned

GraphPad
Xcalibur
GraphPad Prism
TraceFinder

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.

Related Papers

Revue de l'infirmière
Corinne Taéron
Deutsche medizinische Wochenschrift
A El-ArmoucheT Eschenhagen
European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology
K Brøsen, C A Naranjo
Journal of Clinical Psychopharmacology
J WillettsB Beer
© 2022 Meta ULC. All rights reserved