In vitro metabolism of 2,2'-diaminopimelic acid from gram-positive and gram-negative bacterial cells by ruminal protozoa and bacteria.

Applied and Environmental Microbiology
A M Denholm, J R Ling

Abstract

Bacillus megaterium GW1 and Escherichia coli W7-M5 were specifically radiolabeled with 2,2'-diamino[G-3H]pimelic acid [( 3H]DAP) as models of gram-positive and gram-negative bacteria, respectively. These radiolabeled bacterial mutants were incubated alone (control) and with mixed ruminal bacteria or protozoa, and the metabolic processes, rates, and patterns of radiolabeled products released from them were studied. Control incubations revealed an inherent difference between the two substrates; gram-positive supernatants consistently contained 5% radioactivity, whereas even at 0 h, those from the gram-negative mutant released 22%. Incubations with ruminal microorganisms showed that the two mutants were metabolized differently and that protozoa were the major effectors of their metabolism. Protozoa exhibited differential rates of engulfment (150 B. megaterium GW1 and 4,290 E. coli W7-M5 organisms per protozoan per h), and they extensively degraded [3H]DAP-labeled B. megaterium GW1 at rates up to nine times greater than those of ruminal bacteria. By contrast, [3H]DAP-labeled E. coli W7-M5 degradation by either ruminal bacteria or ruminal protozoa was more limited. These fundamental differences in the metabolism of the two mutants, ...Continue Reading

References

Feb 1, 1978·Nihon juigaku zasshi. The Japanese journal of veterinary science·S Imai, K Ogimoto
Sep 1, 1987·The British Journal of Nutrition·R J Wallace, C A McPherson
Dec 1, 1972·Bacteriological Reviews·K H Schleifer, O Kandler
Jun 1, 1969·Journal of General Microbiology·R W White
May 1, 1968·Applied Microbiology·B D Jarvis
Jan 1, 1980·Advances in Parasitology·G S Coleman
Jan 1, 1984·Applied and Environmental Microbiology·C W ForsbergJ G Buchanan-Smith
Sep 1, 1982·The British Journal of Nutrition·R A Leng

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