PMID: 488752Jul 1, 1979Paper

In vitro metabolism of creatinine, methylamine and amino acids by intestinal contents of normal and uraemic subjects

Gut
C W OwensG M Tomlinson

Abstract

An original method which uses in vitro anaerobic incubation at 37 degrees C followed by centrifugation, ultrafiltration, and ion exchange chromatography is described; it shows that faecal material suspended in physiological saline can destroy added creatinine. The rate of breakdown by suspensions from uraemic subjects (mean 780 mumol h-1kg-1 SEM 70) was slightly faster than in normal subjects (mean 550 mumol h-1kg-1 SEM 80). Methylamine concentration increased over eight hours as creatinine was metabolised and sarcosine appeared as an intermediate. The rates of these reactions varied within and between individuals and were inhibited by oxygen and centrifugation but not by oxytetracycline. Concentrations of free amino acids did not change significantly despite the formation of ammonia. This approach should be useful in studying the metabolic inter-relationships between intestinal contents and the host organism in health and disease.

References

Jun 7, 1976·Biochimica Et Biophysica Acta·M Naoi, K Yagi
Sep 1, 1976·Clinical Science and Molecular Medicine·A VinceO M Wrong
Oct 16, 1976·Lancet·M L SimenhoffR W Schaedler
Nov 15, 1975·Experientia·E ten Krooden, C W Owens
May 1, 1979·Clinical Science·C W Owens, W Padovan
Jul 21, 1977·The New England Journal of Medicine·M L SimenhoffR Doty
Dec 1, 1976·The American Journal of Clinical Nutrition·G B Forbes, G J Bruining
Jul 1, 1971·Experientia·P L BalestriM Biagini
Nov 1, 1953·Proceedings of the Society for Experimental Biology and Medicine·F-C CHAO, H TARVER
Mar 1, 1954·Proceedings of the Society for Experimental Biology and Medicine·R GOLDMAN
Oct 1, 1958·Biochimica Et Biophysica Acta·J SZULMAJSTER
Jan 1, 1962·The American Journal of Medicine·P D DOOLANG B THEIL
Oct 8, 1963·Biochimica Et Biophysica Acta·J E NORTONJ R SOKATCH
Mar 1, 1964·The Journal of Clinical Investigation·C D FITCH, D W SINTON
Jan 1, 1964·Scandinavian Journal of Clinical and Laboratory Investigation·E ENGER, E M BLEGEN
Jul 1, 1935·The Journal of Clinical Investigation·J A Shannon

❮ Previous
Next ❯

Citations

Oct 3, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·S C Mitchell, A Q Zhang
Oct 15, 1998·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A Q ZhangR L Smith
Aug 24, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A Q ZhangR L Smith
Jul 14, 2000·Physiological Reviews·M Wyss, R Kaddurah-Daouk
Jan 1, 1986·Annals of Clinical Biochemistry·K Spencer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.