In vitro metabolism of cyadox in rat, chicken and swine using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Haixia WuSuxia Zhang

Abstract

In this work, metabolites of cyadox (CYX) produced by liver microsomes, primary hepatocytes and intestinal microflora systems of rat, chicken and swine were identified and elucidated using ultra-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS). Expected and unexpected metabolites were found out using Metabolynx(XS) software by automatically comparing MS(E) data from the sample and control. The metabolites were reliably characterized by accurate MS/MS spectra and their different fragmentation pathways. In total twenty-four metabolites were identified in the three in vitro systems, fifteen of which were novel. The results revealed the main metabolic pathways of CYX were N→O group reduction, hydroxylation and hydrolysis reactions. Besides, methylation and acetylation reactions which represented phase II drug biotransformations were recognized in the in vitro systems of chicken firstly. It also showed that the metabolic capacity and rapidity of chicken and swine was greater than rat, with some differences in metabolic fate and metabolite varieties. This work contributes to the comprehensive understanding of in vitro metabolism of CYX, and will provide an important basis for furthe...Continue Reading

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Citations

Sep 21, 2013·Journal of Agricultural and Food Chemistry·Yafei LiHuanzhong Ding
Mar 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Lucas Maciel Mauriz MarquesAnderson Rodrigo Moraes de Oliveira
Sep 29, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Lingli HuangZonghui Yuan
Nov 8, 2014·Journal of Veterinary Pharmacology and Therapeutics·X YangY-H Liu
Oct 19, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Bruno A RochaAnderson R M de Oliveira
May 15, 2015·Journal of Agricultural and Food Chemistry·Huang LingliYuan Zonghui
May 14, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Qianying LiuZonghui Yuan
Apr 26, 2018·Frontiers in Pharmacology·Qianying LiuZonghui Yuan
Jan 9, 2020·Journal of Veterinary Pharmacology and Therapeutics·Adeel SattarZonghui Yuan
Oct 12, 2017·Frontiers in Pharmacology·Qianying LiuZonghui Yuan
Sep 7, 2018·Frontiers in Pharmacology·Qianying LiuZonghui Yuan
Jun 14, 2021·Regulatory Toxicology and Pharmacology : RTP·Sechenchogt HarnudLingli Huang
May 17, 2019·Journal of Agricultural and Food Chemistry·Huiling TanLingli Huang

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