In Vitro Methods to Study Colon Release: State of the Art and An Outlook on New Strategies for Better In-Vitro Biorelevant Release Media

Pharmaceutics
Marie WahlgrenBetty Lomstein Pedersen

Abstract

The primary focus of this review is a discussion regarding in vitro media for colon release, but we also give a brief overview of colon delivery and the colon microbiota as a baseline for this discussion. The large intestine is colonized by a vast number of bacteria, approximately 1012 per gram of intestinal content. The microbial community in the colon is complex and there is still much that is unknown about its composition and the activity of the microbiome. However, it is evident that this complex microbiota will affect the release from oral formulations targeting the colon. This includes the release of active drug substances, food supplements, and live microorganisms, such as probiotic bacteria and bacteria used for microbiota transplantations. Currently, there are no standardized colon release media, but researchers employ in vitro models representing the colon ranging from reasonable simple systems with adjusted pH with or without key enzymes to the use of fecal samples. In this review, we present the pros and cons for different existing in vitro models. Furthermore, we summarize the current knowledge of the colonic microbiota composition which is of importance to the fermentation capacity of carbohydrates and suggest a s...Continue Reading

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Citations

Jan 31, 2021·Food Chemistry·Yunbing Tan, David Julian McClements
Feb 4, 2021·Pharmaceutics·Glenn LemmensPatrick Augustijns
Jul 27, 2021·Frontiers in Bioengineering and Biotechnology·Kangkang YingBinbin Xie
Aug 15, 2021·Advanced Drug Delivery Reviews·Connor O'FarrellHannah Batchelor

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Methods Mentioned

BETA
PCR
biopsies

Software Mentioned

InfoGest
SHIME

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