In vitro mineralization studies with substrate-immobilized bone morphogenetic protein peptides

The Journal of Oral Implantology
Keith KirkwoodKay C Dee

Abstract

Understanding the factors that control osteoblastic behavior is centrally important in establishment of successful osseointegration. Pharmacogenetic control of the osteoblast to increase the mineral content around dental implants may offer a unique advantage to clinicians in improving osseointegration success and decreasing time before mechanical loading. This in vitro pilot study has screened for bioactive peptides derived from bone morphogenetic protein 7 (BMP-7) (also called osteogenic protein 1 [OP-1]). Thirteen overlapping peptides of BMP-7 were synthesized and covalently coupled to glass coverslip substrates using silane chemistry. The rate and relative amount of mineralization were compared by von Kossa analysis using primary rat calvarial osteoblastic cell populations during a 7- to 21-day period. In addition, bone sialoprotein (BSP) and osteocalcin (OC) gene expression was measured from osteoblastic cells grown on peptide-immobilized glass coverslips by reverse transcriptase-polymerase chain reaction. Initial results from mineralization studies suggested the BMP-7-derived peptides were able to support mineralization to varying degrees with enhanced peptide-induced mineralization from the C-and N-termini of the BMP-7 mo...Continue Reading

Citations

Apr 30, 2013·Journal of Biomedical Materials Research. Part a·Kazuaki YuasaTakashi Inoue
Oct 13, 2004·Journal of Biomedical Materials Research. Part a·Vassilis KarageorgiouDavid Kaplan
Sep 17, 2005·Clinical Oral Implants Research·Henning SchliephakeAndreas Sewing
Jun 30, 2007·Journal of Biomedical Materials Research. Part a·James E HoKevin E Healy
Apr 22, 2011·Macromolecular Bioscience·Kristyn S Masters
Aug 2, 2006·Molecular BioSystems·David M Salvay, Lonnie D Shea
Nov 5, 2008·Journal of Biomedical Materials Research. Part a·Yupeng Chen, Thomas J Webster
Oct 17, 2008·The International Journal of Artificial Organs·Z L ShiW Wang
Apr 15, 2021·Molecular Therapy. Methods & Clinical Development·Marjolein M J CaronTim J M Welting

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