In-vitro model for bacterial growth inhibition of compartmentalized infection treated by an ultra-high concentration of antibiotics.

PloS One
Moris TopazSarit Freimann

Abstract

Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli), and Staphylococcus aureus (S. aureus) are common pathogens encountered in infected cardiovascular-implantable electronic device (CIED). Continuous, in-situ targeted, ultra-high concentration antibiotic (CITA) treatment is a novel antibiotic treatment approach for localized infections. CITA provides sufficient local antibiotic concentrations to heavily infected cavities while avoiding systemic toxicity. In-vitro confirmation of the efficacy of the CITA treatment approach in simulated compartmentalized infections. A rapid automated bacterial culture analyzing system) Uro4 HB&L™ (was applied to compare the efficacy of selected antibiotics at a standard minimal inhibitory concentration (1MIC), 4MIC, and CITA at 103MIC, for growth inhibition of high bacterial loads (106 colony-forming-units/ml) of ATCC strains of P. aeruginosa, E. coli, and S. aureus. The addition of gentamicin and amikacin at 1MIC concentrations only temporarily inhibited the exponential growth of E. coli and P. aeruginosa. 4MIC level extended the delay of exponential bacterial growth. Increasing concentrations of vancomycin similarly temporarily delayed S. aureus growth. All tested antibiotics at ...Continue Reading

References

Oct 1, 1989·Antimicrobial Agents and Chemotherapy·H AnwarJ W Costerton
Oct 28, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·James H Jorgensen, Mary Jane Ferraro
Jul 13, 2011·Current Cardiology Reports·John Rickard, Bruce L Wilkoff
Feb 9, 2012·FEMS Immunology and Medical Microbiology·Werner Zimmerli, Claus Moser
Aug 31, 2012·The New England Journal of Medicine·Larry M BaddourWalter R Wilson
Nov 12, 2015·World Journal of Critical Care Medicine·Paolo CotogniMauro Rinaldi
Oct 18, 2016·Journal of Wound, Ostomy, and Continence Nursing : Official Publication of the Wound, Ostomy and Continence Nurses Society·Laura E EdsbergMary Sieggreen
Sep 9, 2017·Journal of the American College of Surgeons·Peter J NicksicLuke G Gutwein
Sep 19, 2017·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Fred M KusumotoOussama Wazni
Mar 20, 2018·The Journal of Antimicrobial Chemotherapy·G F DallM P Gallagher
Apr 25, 2018·Nature Communications·Erik Wistrand-YuenDan I Andersson
Jul 10, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Darren WongBrad Spellberg
Nov 13, 2018·Arrhythmia & Electrophysiology Review·Adryan A PerezRoger G Carrillo
Mar 19, 2019·The New England Journal of Medicine·Khaldoun G TarakjiUNKNOWN WRAP-IT Investigators
Jun 19, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Beatriz AlonsoMaría Guembe
Sep 19, 2019·General Thoracic and Cardiovascular Surgery·Antonio NennaCristiano Spadaccio

❮ Previous
Next ❯

Software Mentioned

GraphPad Prism
GraphPad
CITA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.