In vitro model for contact sensitization: I. Stimulatory capacities of human blood-derived dendritic cells and their phenotypical alterations in the presence of contact sensitizers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
J DegwertL H Kligman

Abstract

Dendritic cells (DC) are highly specialized antigen-presenting cells (APC) located in many non-lymphoid tissues and a specialized form of DC-the Langerhans cell (LC)-is found in the skin. The functionality of LC as APC is crucial for the induction of an allergic contact dermatitis. For a long time LC research has been hampered by the limiting numbers of functionally active LC that could be isolated from human skin. The addition of GM-CSF and IL-4 to the non-adherent fraction of mononuclear cells from peripheral blood generated a large amount of CD1a(+) HLA-DR(+) DC. These in vitro-generated DC exhibited the morphology, phenotype and autologous T-lymphocyte stimulating capacity of the human DC/LC system. We had tested phenotypical alterations of in vitro-generated DC under the influence of subtoxic concentrations of different chemicals and contact sensitizers. In vitro stimulation with the contact sensitizers urushiol, primin, C10-and C11-primin analogues, alantolactone, isoalantolactone and NiSO(4) resulted in a decrease of HLA-DR expression on the surface of these cells if the incubation period did not exceed 3 hr. Incubation with irritants like sodium lauryl sulfate (SLS) and benzalkonium chloride did not change or increase t...Continue Reading

References

Apr 1, 1991·Immunology Today·T J Braciale, V L Braciale
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A H Enk, S I Katz
Jan 1, 1986·Archives of Dermatological Research·R E SchopfK Bork
Jan 1, 1980·Immunological Reviews·G StinglS I Katz
Jul 1, 1994·The Journal of Experimental Medicine·N RomaniG Schuler

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May 12, 2005·Toxicology and Applied Pharmacology·Ben C HuletteG Frank Gerberick
May 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M J StaquetJ Péguet-Navarro
Oct 18, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H TuschlE Weber
Mar 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C A RyanG F Gerberick
Nov 9, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T AshikagaS Aiba
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D J BenfordJ Southee
May 12, 2007·Toxicology and Applied Pharmacology·Cindy A RyanG Frank Gerberick
Jun 15, 2006·Journal of Applied Toxicology : JAT·Ian R JowseyIan Kimber
Mar 15, 2005·Toxicology Letters·Ann C A De SmedtGreet E R Schoeters
Jul 26, 2005·Toxicology and Applied Pharmacology·Philippe AzamJean-Claude Ourlin
Aug 26, 2010·Journal of Immunotoxicology·Valentina GalbiatiEmanuela Corsini
Jun 7, 2012·Cutaneous and Ocular Toxicology·Ian KimberDavid A Basketter
Apr 15, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hendrik ReuterAndreas Schepky
Jul 15, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cindy A RyanIan Kimber
Oct 30, 2008·Journal of Immunotoxicology·Alessandra GennariLaura Gribaldo
Apr 29, 1998·The Journal of Urology·R RamonerM Thurnher
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·David BasketterAndrew Worth
Jan 7, 2020·Alternatives to Laboratory Animals : ATLA·Bianca MariglianiAnna Maria Buehler
Oct 8, 2009·Alternatives to Laboratory Animals : ATLA·Emanuela Corsini, Erwin L Roggen
Apr 6, 2005·Alternatives to Laboratory Animals : ATLA·Silvia CasatiUNKNOWN Report and Recommendations of ECVAM Workshop 51

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