In vitro model for contact sensitization: II. Induction of IL-1beta mRNA in human blood-derived dendritic cells by contact sensitizers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
K ReutterU Hoppe

Abstract

Epidermal mRNA for interleukin 1beta (IL-1beta) has been shown to be increased following exposure of mouse skin to sensitizing compounds. In addition, this early upregulation of IL-1beta was specific for contact sensitizers, while expression of IL-1beta was unaffected by irritants. Langerhans cells are the major source of IL-1beta within the epidermis in the induction phase of skin sensitization. Since the isolation of Langerhans cells from skin biopsies results only in low frequencies, we decided to use dendritic cells (DCs) generated from peripheral blood as Langerhans cell equivalents to investigate the ability of five contact sensitizers and one irritant to induce IL-1beta gene expression in vitro. For our studies we cultivated DCs in serum-free medium supplemented with granulocyte/macrophage-colony stimulation factor (GM-CSF) and interleukin 4 (IL-4). The DCs showed a typical dendritic morphology, a characteristic expression of surface markers and high stimulatory capacity for autologous T cells. 5-day-old DCs were incubated with subtoxic concentrations of the contact sensitizers pentadecyl-catechol, 2,4,6-trinitrobenezene sulfonic acid, 2,4-dinitrofluorobenzene, NiSO(4), K(2)Cr(2)O(7) and the irritant sodium dodecyl sulfa...Continue Reading

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Citations

May 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M J StaquetJ Péguet-Navarro
Jun 1, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V FacyR Schmidt
Jul 14, 1999·Toxicology Letters·I KimberR J Dearman
Jul 25, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J S PichowskiI Kimber
Oct 18, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H TuschlE Weber
Mar 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C A RyanG F Gerberick
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I KimberR J Dearman
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H Tuschl, R Kovac
May 22, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·W D Pennie, I Kimber
Nov 9, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T AshikagaS Aiba
Mar 26, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Y YoshidaH Suzuki
Feb 6, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I KimberR J Dearman
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