Abstract
Drugs for the treatment and prevention of nervous system diseases must permeate the blood-brain barrier to take effect. In vitro models of the blood-brain barrier are therefore important in the investigation of drug permeation mechanisms. However, to date, no unified method has been described for establishing a blood-brain barrier model. Here, we modified an in vitro model of the blood-brain barrier by seeding brain microvascular endothelial cells and astrocytes from newborn rats on a polyester Transwell cell culture membrane with 0.4-µm pores, and conducted transepithelial electrical resistance measurements, leakage tests and assays for specific blood-brain barrier enzymes. We show that the permeability of our model is as low as that of the blood-brain barrier in vivo. Our model will be a valuable tool in the study of the mechanisms of action of neuroprotective drugs.
Citations
Jul 5, 2016·International Journal of Pharmaceutics·Lin WeiJian-Cheng Wang
Jul 6, 2016·Cellular and Molecular Neurobiology·Guang YangXinxin Piao
Sep 16, 2018·Annals of Biomedical Engineering·Navein ArumugasaamyJohn P Fisher
Mar 12, 2019·Journal of Cardiovascular Pharmacology·Sipeng WuHuazheng Liang
Jul 7, 2017·Immunology and Cell Biology·Sandip Ashok SonarGirdhari Lal
Feb 15, 2017·Experimental Biology and Medicine·Duc Tt PhanChristopher Cw Hughes
Jul 10, 2018·Molecular Neurobiology·T Peeyush KumarSpiros L Blackburn
Jun 28, 2019·Frontiers in Cellular Neuroscience·Nicole L StoneSaoirse E O'Sullivan
Apr 2, 2020·Drug Delivery·Yang HanBaoshan Sun
May 15, 2020·Journal of Nanobiotechnology·Chunhong GaoYang Yang
Jun 6, 2018·Inflammopharmacology·Sarah BenchabaneChafia Touil-Boukoffa
Jun 12, 2018·Biomicrofluidics·Cynthia HajalRoger D Kamm
Nov 21, 2018·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Hui LiuWei Lu
May 7, 2019·Nuclear Medicine and Molecular Imaging·Do Won Hwang
Apr 7, 2020·Acta Biomaterialia·Chunhong GaoChunsheng Gao
Apr 17, 2021·Oxidative Medicine and Cellular Longevity·Qin ZhangRongxue Wu
May 19, 2018·Brain Research Bulletin·Guang YangHuangzheng Liang
Apr 18, 2021·Translational Oncology·Robin AugustineAnwarul Hasan
Oct 1, 2020·Bioactive Materials·Yang HanBaoshan Sun
Jun 26, 2021·ACS Biomaterials Science & Engineering·Christos Panagiotis TasiopoulosMy Hedhammar
Jun 29, 2021·Tissue Engineering and Regenerative Medicine·Quan-You LiGuang-Zhen Jin
Sep 8, 2021·Signal Transduction and Targeted Therapy·Ling ZhangChuan Qin