In vitro model of the blood-brain barrier established by co-culture of primary cerebral microvascular endothelial and astrocyte cells

Neural Regeneration Research
Yan WangXing-Xing Piao

Abstract

Drugs for the treatment and prevention of nervous system diseases must permeate the blood-brain barrier to take effect. In vitro models of the blood-brain barrier are therefore important in the investigation of drug permeation mechanisms. However, to date, no unified method has been described for establishing a blood-brain barrier model. Here, we modified an in vitro model of the blood-brain barrier by seeding brain microvascular endothelial cells and astrocytes from newborn rats on a polyester Transwell cell culture membrane with 0.4-µm pores, and conducted transepithelial electrical resistance measurements, leakage tests and assays for specific blood-brain barrier enzymes. We show that the permeability of our model is as low as that of the blood-brain barrier in vivo. Our model will be a valuable tool in the study of the mechanisms of action of neuroprotective drugs.

Citations

Sep 16, 2018·Annals of Biomedical Engineering·Navein ArumugasaamyJohn P Fisher
Jul 7, 2017·Immunology and Cell Biology·Sandip Ashok SonarGirdhari Lal
Jul 10, 2018·Molecular Neurobiology·T Peeyush KumarSpiros L Blackburn
Jun 28, 2019·Frontiers in Cellular Neuroscience·Nicole L StoneSaoirse E O'Sullivan
Nov 21, 2018·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Hui LiuWei Lu
May 7, 2019·Nuclear Medicine and Molecular Imaging·Do Won Hwang
Apr 17, 2021·Oxidative Medicine and Cellular Longevity·Qin ZhangRongxue Wu
Apr 18, 2021·Translational Oncology·Robin AugustineAnwarul Hasan
Jun 26, 2021·ACS Biomaterials Science & Engineering·Christos Panagiotis TasiopoulosMy Hedhammar
Jun 29, 2021·Tissue Engineering and Regenerative Medicine·Quan-You LiGuang-Zhen Jin

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Methods Mentioned

BETA
enzyme linked immunosorbent assay

Software Mentioned

SPSS

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