In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure

Methods in Enzymology
Christopher ArnetteKathleen J Green

Abstract

Much of our understanding of the biological processes that underlie cellular functions in humans, such as cell-cell communication, intracellular signaling, and transcriptional and posttranscriptional control of gene expression, has been acquired from studying cells in a two-dimensional (2D) tissue culture environment. However, it has become increasingly evident that the 2D environment does not support certain cell functions. The need for more physiologically relevant models prompted the development of three-dimensional (3D) cultures of epithelial, endothelial, and neuronal tissues (Shamir & Ewald, 2014). These models afford investigators with powerful tools to study the contribution of spatial organization, often in the context of relevant extracellular matrix and stromal components, to cellular and tissue homeostasis in normal and disease states.

Citations

Jan 22, 2017·The Journal of Investigative Dermatology·Robert B Hamanaka, Gökhan M Mutlu
Mar 6, 2017·The Journal of Investigative Dermatology·Mrinal K SarkarJohann E Gudjonsson
Jun 7, 2017·Biofabrication·Byoung Soo KimDong-Woo Cho
Sep 29, 2019·Pigment Cell & Melanoma Research·Christopher R ArnetteKathleen J Green
Aug 26, 2018·Scientific Reports·Michaela Zorn-KruppaJohanna M Brandner
Sep 11, 2020·Acta Biomaterialia·Vincenza De GregorioGiorgia Imparato
Aug 20, 2019·The Journal of Investigative Dermatology·Haoming LiuAmy S Paller
Aug 30, 2019·The Journal of Investigative Dermatology·Eran Cohen-BarakKathleen J Green
Apr 18, 2021·The Journal of Investigative Dermatology·Shuai ShaoJohann E Gudjonsson
Jun 2, 2021·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Kameel ZunigaMarissa Nichole Rylander
Jun 12, 2021·The Journal of Investigative Dermatology·Julia LachnerLeopold Eckhart

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