PMID: 16511984Mar 4, 2006Paper

In vitro models for studying trophoblast transcellular transport

Methods in Molecular Medicine
Claudia J BodeKenneth L Audus

Abstract

In vitro models have proven to be effective in studying the placental transporters that play a role in the exchange of nutrients, waste products, and drugs between the maternal and fetal circulations. Although primary cultures of trophoblast cells can be used to perform uptake, efflux, and metabolism studies, only the rodent HRP-1 and the human BeWo cell lines have been shown to form confluent monolayers when grown on semi-permeable membranes. Protocols for the revival, maintenance, passage, and growth of BeWo cells for transporter expression and transcellular transport studies are provided.

Citations

Apr 20, 2007·American Journal of Physiology. Cell Physiology·Marc U BaumannNicholas P Illsley
Aug 11, 2020·Bio-design and Manufacturing·Maria E Zubizarreta, Shuo Xiao
Sep 26, 2020·Scientific Reports·Anna-Elisabeth KreuderLutz Kloke
Jun 20, 2020·ACS Applied Materials & Interfaces·Christina M Bailey-HytholtAnita Shukla

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