In vitro modulation of cartilage shape plasticity by biochemical regulation of matrix remodeling.

Tissue Engineering. Part a
Gregory M Williams, Robert L Sah

Abstract

With consideration of the need for cartilage grafts of specific sizes and shapes in orthopedics and other fields, immature cartilage explants and grafts have recently been molded in vitro and in vivo. Nonsurgical correction of cartilage deformities and malformations often uses mechanical stimuli and further demonstrates the plasticity of cartilage shape. Cartilage shape plasticity appears to diminish with maturation, coincident with changes in matrix composition. This study's objectives were to characterize shape plasticity of articular cartilage from immature and mature bovines and test whether altering proteoglycan and collagen (COL) remodeling modulates shape plasticity in vitro. Cartilage explants were analyzed fresh on day 0 or after 14 days of culture in the presence of β-D-xyloside to suppress glycosaminoglycan accumulation or β-aminopropionitrile (BAPN) to inhibit lysyl oxidase-mediated COL crosslinking. Culture with β-d-xyloside and BAPN differentially regulated cartilage size, composition, and shape plasticity, with an inverse association between shape plasticity and the ratio of tissue COL to glycosaminoglycan. Retention of a mechanically imposed contour was increased by culture with BAPN compared to day 0 calf carti...Continue Reading

References

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Citations

Aug 23, 2012·Histochemistry and Cell Biology·Siyuan LiClare E Hughes
Feb 22, 2013·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Marsha S ReutherDeborah Watson
Feb 20, 2016·Angewandte Chemie·Falguni PatiHelene Andersson Svahn
Oct 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Eleftherios A MakrisKyriacos A Athanasiou

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Methods Mentioned

BETA
glycosylation

Software Mentioned

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