PMID: 11315097Apr 21, 2001Paper

In vitro modulation of human lung cancer cell line invasiveness by antisense cDNA of tissue factor pathway inhibitor-2

Clinical & Experimental Metastasis
S S LakkaJ S Rao

Abstract

Human tissue factor pathway inhibitor-2 (TFPI-2) is a Kunitz-type serine protease inhibitor that inhibits plasmin, trypsin, chymotrypsin, cathepsin G and plasma kallikrein but not urokinase (uPA) or tissue-type plasminogen activator and thrombin. Earlier studies from our and other laboratories have shown that the production of TFPI-2 is downregulated during the progression of various cancers. To investigate the role of TFPI-2 in the invasion and metastasis of lung tumors, the human lung cancer cell line A549, which produces high levels of TFPI-2, was stably transfected with a vector capable of expressing an antisense transcript complementary to the full-length TFPI-2 mRNA. Northern blot analysis was used to quantify the TFPI-2 mRNA transcript, and western blot analysis was used to measure TFPI-2 protein levels in parental cells and stably transfected (vector and antisense) clones. The levels of TFPI-2 mRNA and protein were significantly less in antisense clones than in the parental and vector controls. The invasive potential of the parental cells and stably transfected vector clones in vitro, as measured by the Matrigel invasion assay, was also markedly less than that of antisense clones. Further characterization of these clone...Continue Reading

Citations

Nov 5, 2011·Journal of Pediatric Hematology/oncology·Pan JianNi Jian
Feb 18, 2003·The Journal of Biological Chemistry·Travis Dunckley, Ronald J Lukas
Mar 10, 2009·Clinical & Experimental Metastasis·Sophie IochmannPascale Reverdiau
Apr 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chun-Ming WongIrene Oi-Lin Ng
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Koshiki MinoTamio Mizukami
Mar 21, 2007·International Journal of Cancer. Journal International Du Cancer·Yoshimasa NobeyamaToshikazu Ushijima
Oct 27, 2004·Biochemical and Biophysical Research Communications·Desheng KongHouyan Song

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