In vitro modulation of tumor necrosis factor α production in THP-1 cells by lactic acid bacteria isolated from healthy human infants

Anaerobe
Boonyarut LaddaMalai Taweechotipatr

Abstract

The human microbiota is a source of probiotics capable of modulating the host immune system. In this study, we collected fecal samples from 100 healthy infants and isolated lactic acid bacteria which were screened for immune modulating effects on tumor necrosis factor α (TNF-α) production. Cell-free culture supernatants from 26 isolates were able to decrease TNF-α production in vitro and three of the isolates were selected as candidate probiotics (MSMC39-1, MSMC39-3, MSMC57-1). These isolates were identified using 16S ribosomal DNA sequencing as Lactobacillus paracasei, Lactobacillus casei, and Weissella confusa respectively. All three isolates were acid tolerant and bile tolerant to pH 3.0 and 4% bile respectively. Preparations of cell-free culture supernatants were processed and tested, and revealed that cell-free culture supernatants of isolates L. paracasei MSMC39-1, L. casei MSMC39-3, and W. confusa MSMC57-1 decreased the production of TNF-α significantly and were heat resistant. Only L. paracasei MSMC39-1 supernatant was proteinase-K sensitive. The effects of viable bacteria, heat-killed bacteria, and sonicated bacteria were compared. The heat-killed preparations of isolate W. confusa MSMC57-1 decreased the production of ...Continue Reading

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Citations

Mar 13, 2014·PloS One·Alexandria N ArdissoneJosef Neu
May 30, 2020·Probiotics and Antimicrobial Proteins·Chittapon JantararussameeWisuit Pradidarcheep
Aug 28, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Maged S Bin MasalamRashad R Al-Hindi

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