PMID: 1845879Jan 1, 1991Paper

In vitro mRNA degradation system to study the virion host shutoff function of herpes simplex virus

Journal of Virology
C R Krikorian, G S Read

Abstract

The virion host shutoff (vhs) gene of herpes simplex virus encodes a virion polypeptide that induces degradation of host mRNAs at early times and rapid turnover of viral mRNAs throughout infection. To better investigate the vhs function, an in vitro mRNA degradation system was developed, consisting of cytoplasmic extracts from HeLa cells infected with wild-type herpes simplex virus type 1 or a mutant encoding a defective vhs polypeptide. Host and viral mRNAs were degraded rapidly in extracts from cells productively infected with wild-type herpes simplex virus type 1 but not in extracts from mock-infected cells or cells infected with the mutant vhs1. In contrast, 28S rRNA was stable in all three kinds of extract. Accelerated turnover of host mRNAs was also observed in extracts from cells infected with wild-type virus in the presence of dactinomycin, indicating that the activity was induced by a structural component of the infecting virions. The in vitro vhs activity was inactivated by heat or proteinase K digestion but was insensitive to brief treatment of the extracts with micrococcal nuclease. It was not inhibited by placental RNase inhibitor, it exhibited a strong dependence upon added Mg2+, it was active at concentrations of...Continue Reading

Citations

Mar 16, 2005·Journal of Virology·Wei-Li HsuJames R Smiley
Dec 15, 2005·Journal of Virology·Paul T Sobol, Karen L Mossman
Mar 1, 1996·Nucleic Acids Research·J Hua, V Paetkau
Aug 1, 2013·Wiley Interdisciplinary Reviews. RNA·G Sullivan Read
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Brunella TaddeoBernard Roizman
Nov 13, 2013·Nucleic Acids Research·Giuliano GiulianiCatherine Rabouille
Jan 9, 2016·The FEBS Journal·Ya-Fen LiuWei-Li Hsu
Jul 16, 2010·Journal of Virology·Brunella TaddeoBernard Roizman
Jun 12, 2020·Seminars in Cell & Developmental Biology·William RodriguezMandy Muller
Dec 31, 2020·Viruses·Christos DogrammatzisMaria Kalamvoki
May 1, 1996·Trends in Genetics : TIG·J Ross
Sep 1, 1995·Microbiological Reviews·J Ross
Dec 10, 1999·Journal of the American Society of Nephrology : JASN·C YalcindagT Naveh-Many

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