In vitro mutagenesis of Bacillus subtilis by using a modified Tn7 transposon with an outward-facing inducible promoter.

Applied and Environmental Microbiology
Christophe BordiJohn D Helmann

Abstract

A Tn7 donor plasmid, pTn7SX, was constructed for use with the model gram-positive bacterium Bacillus subtilis. This new mini-Tn7, mTn7SX, contains a spectinomycin resistance cassette and an outward-facing, xylose-inducible promoter, thereby allowing for the regulated expression of genes downstream of the transposon. We demonstrate that mTn7SX inserts are obtained at a high frequency and occur randomly throughout the B. subtilis genome. The utility of this system was demonstrated by the selection of mutants with increased resistance to the antibiotic fosfomycin or duramycin.

References

Jun 1, 1992·Journal of Bacteriology·P B Vander Horn, S A Zahler
Dec 1, 1990·Journal of Bacteriology·M A PetitS D Ehrlich
Apr 1, 1989·Molecular & General Genetics : MGG·J Casadesus, J R Roth
Aug 1, 1986·Journal of Bacteriology·M A Vandeyar, S A Zahler
Aug 14, 1972·Journal of Molecular Biology·E DaniellJ Abelson
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P J YoungmanR Losick
Apr 19, 1996·Science·E W May, N L Craig
Feb 1, 1997·Molecular Microbiology·B Rutberg
Jan 10, 1998·The EMBO Journal·A E Stellwagen, N L Craig
Jan 1, 1997·Microbial & Comparative Genomics·K E NelsonB A Dougherty
May 16, 2000·Journal of Bacteriology·R T DeBoy, N L Craig
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·C M SassettiE J Rubin
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·J E Peters, N L Craig
Jun 21, 2002·Methods in Enzymology·Nurjana BachmanNancy L Craig
Nov 12, 2002·Journal of Bacteriology·Ahmed GaballaJohn D Helmann
Apr 16, 2003·Veterinary Microbiology·J D SlaterD J Maskell
Apr 16, 2003·Genome Research·Irene CastanoBrendan P Cormack
Nov 26, 2003·Antimicrobial Agents and Chemotherapy·Stephen J SalipanteBarry G Hall
Dec 4, 2003·Molecular Microbiology·Thorsten MascherJohn D Helmann
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dipankar MannaN Patrick Higgins
Aug 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Taeok BaeDominique M Missiakas
Sep 24, 2004·Research in Microbiology·M Joanne LemieuxDa-Neng Wang
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Phyllis I Hanson, Sidney W Whiteheart
Jan 5, 2006·Applied and Environmental Microbiology·Yoann Le BretonW G Haldenwang
Mar 15, 2006·Plasmid·Christina TamDominique Missiakas
Feb 20, 2007·Applied and Environmental Microbiology·Min CaoHélène Marquis
May 8, 2007·Biophysical Journal·Kunihiko IwamotoToshihide Kobayashi
Oct 13, 2007·Journal of Microbiological Methods·Adam C WilsonJames A Hoch

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Citations

Sep 6, 2011·Nature Chemical Biology·Hao WangTimothy C Meredith
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·Yong Heon LeeJohn D Helmann
Oct 25, 2012·Mobile Genetic Elements·Timothy C MeredithTerry Roemer
Jun 26, 2013·Antimicrobial Agents and Chemotherapy·Yong Heon Lee, John D Helmann

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