PMID: 9008720Dec 20, 1996Paper

In vitro mutagenicity and genotoxicity study of 1,2-dichloroethylene, 1,1,2-trichloroethane, 1,3-dichloropropane, 1,2,3-trichloropropane and 1,1,3-trichloropropene, using the micronucleus test and the alkaline single cell gel electrophoresis technique (comet assay) in human lymphocytes

Mutation Research
M Tafazoli, M Kirsch-Volders

Abstract

The main objective of this study was to compare the cytotoxic genotoxic and mutagenic activity of a number of chlorinated aliphatic hydrocarbons, which are widely used as chemical intermediates, solvents, degreasing agents etc. in industry, and to establish the structure-toxicity relationship of the chemicals by using the most adequate determinants in estimating their toxicity. The mutagenicity and cytotoxicity of some of the candidate chemicals, namely 1,2-dichloroethylene, 1,1,2-trichloroethane, 1,3-dichloropropane, 1,2,3-trichloropropane and 1,1,3-trichloropropene were evaluated in an in vitro micronucleus assay. The cytokinesis-block methodology was applied on human lymphocytes in the presence or absence of an external metabolic activation system (S9-mix). In the micronucleus assay, all test substances, except 1,2,3-trichloropropane with and without S9-mix and 1,1,2-trichloroethane without S9-mix in the repeated experiment, exhibited a low but statistically significant mutagenic activity, compared to the concurrent control. However, none of the five chemicals was able to induce a clear and reproducible linear dose-dependent increase in micronucleus frequencies in this assay. Generally, mutagenic activity of the chemicals wa...Continue Reading

References

Dec 1, 1977·Environmental Health Perspectives·E K Weisburger
Mar 1, 1992·Toxicology and Applied Pharmacology·G L Weber, I G Sipes
Jan 1, 1988·Environmental and Molecular Mutagenesis·F Ratpan, H Plaumann
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Jan 1, 1987·Environmental Mutagenesis·W von der HudeA Basler
Feb 1, 1985·Mutation Research·M Fenech, A A Morley
Jan 1, 1984·Teratogenesis, Carcinogenesis, and Mutagenesis·G BronzettiJ Crenshaw
Sep 15, 1980·Toxicology and Applied Pharmacology·F P GuengerichP G Watanabe
Jan 1, 1980·Environmental Mutagenesis·S J Stolzenberg, C H Hine
May 1, 1982·Toxicology Letters·A B DiRenzoI G Sipes
Jan 1, 1981·Carcinogenesis·K M Ivanetich, L H Van den Honert
Jan 1, 1995·Environmental and Molecular Mutagenesis·G L ErexsonA D Kligerman
May 1, 1995·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·R D IrwinS L Eustis
Jul 1, 1993·Mutation Research·V J McKelvey-MartinA Collins
Feb 1, 1995·Mutation Research·D W FairbairnK L O'Neill
Apr 1, 1994·Regulatory Toxicology and Pharmacology : RTP·H S RosenkranzG Klopman
Feb 15, 1994·Toxicology Letters·C Dees, C Travis
Jan 1, 1993·Critical Reviews in Toxicology·J L RaucyJ M Lasker

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Citations

Jul 10, 2003·International Journal of Toxicology·Charles E HealyMark A Martens
Jan 8, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Rogelio Tornero-VelezMarina V Evans
Dec 24, 2003·Teratogenesis, Carcinogenesis, and Mutagenesis·Elizabeth D WagnerMichael J Plewa
May 19, 2006·International Journal of Environmental Research and Public Health·Babu P PatlollaPaul B Tchounwou
Jul 24, 2001·Pediatrics International : Official Journal of the Japan Pediatric Society·R DündarözV Baltaci
Jan 1, 2007·Toxicology Mechanisms and Methods·Jing J WangBarbara J S Sanderson
Mar 7, 2003·Mutagenesis·Marlies De BoeckMicheline Kirsch-Volders
Jan 29, 2014·Sleep & Breathing = Schlaf & Atmung·Jing XieTieliang Ma
Sep 7, 2017·Environmental Science and Pollution Research International·Iman Al-SalehPulicat Manogaran
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·E RojasM Valverde
Jun 22, 2018·Ecotoxicology and Environmental Safety·Alfredo SantovitoPiero Cervella

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