In vitro neuraminidase inhibitory concentration (IC50 ) of four neuraminidase inhibitors in the Japanese 2016-17 season: Comparison with the 2010-11 to 2015-16 seasons

Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy
Hideyuki IkematsuKazuhito Shiosakai

Abstract

To assess the extent of susceptibility to the four most commonly used neuraminidase inhibitors (NAIs) in the viruses epidemic in the 2016-17 Japanese influenza season, we measured the 50% inhibitory concentration (IC50) of these NAIs for influenza virus isolates from patients and compared them with the results from the 2010-11 to 2015-16 seasons. Viral isolation was done with specimens obtained prior to treatment, and the type and subtype was determined by RT-PCR using type- and subtype-specific primers. The IC50 was determined by a neuraminidase inhibition assay using a fluorescent substrate. A total of 276 virus isolates, 6 A (H1N1)pdm09 (2.2%), 249 A (H3N2) (90.2%), and 21 B (7.6%), had the IC50 measured for the four NAIs. B isolates included 11 (52.4%), 9 (42.9%), and one (4.8%) of the Victoria, Yamagata, and undetermined strains, respectively. No A (H1N1)pdm09 with highly reduced sensitivity for oseltamivir was found in the 2016-17 season. No isolate with highly reduced sensitivity to the four NAIs have been found for A (H3N2) or B from the 2010-11 to 2016-17 seasons. No significant trend of increase or decrease was found in the geometric mean IC50s of the four NAIs during the seven studied seasons. These results indicate ...Continue Reading

Citations

Sep 16, 2018·Viruses·Ahmed MostafaStephan Pleschka
Dec 15, 2018·Influenza and Other Respiratory Viruses·Yong ChongHideyuki Ikematsu
Jul 7, 2021·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Takashi NakanoYomei Matsuoka

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