PMID: 9179773Jun 1, 1997Paper

In vitro neutralization of hepatitis B virus by monoclonal antibodies against the viral surface antigen

Journal of Medical Virology
C J RyuH J Hong

Abstract

In vitro HBV infection and neutralization were assayed using an anti-preS1 murine monoclonal antibody (1B3) and anti-preS2 (H69K) and anti-S (CS131A) murine-human chimeric antibodies. The 1B3 (IgG1) and H69K (IgG1) was constructed previously and the CS131A was constructed for this study by expressing stably the chimeric heavy and light chains in Chinese hamster ovary cells and purifying from the culture supernatant. Previous study showed that the H69K and CS131A recognize known virus-neutralizing epitopes, while the 1B3 does not. For the assays, adult human hepatocyte primary culture was infected with the adr or ayw subtype of HBV, and the infectivity and subsequent replication was confirmed both by measuring the kinetics of HB-sAg secretion by the infected cells and detecting the intermediate replicative form of HBV DNA in the cells. Next, the hepatocytes were infected with the adr or ayw subtype of the virus that had been preincubated with various concentrations of each of the antibodies and the neutralization of HBV was analyzed. The results showed that the anti-preS2 and anti-S chimeric antibodies exhibited neutralizing activity against both the adr and ayw subtypes of the virus, with approximately 1,000 and 2,000 times hig...Continue Reading

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Citations

Nov 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Philippe GriponChristiane Guguen-Guillouzo
Aug 14, 2003·Journal of Virology·Dieter GlebeWolfram H Gerlich
Aug 13, 2015·International Journal of Molecular Sciences·Akinobu TakakiTakahito Yagi
Apr 17, 2016·Journal of Hepatology·Antonio Bertoletti, Carlo Ferrari
Apr 22, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Gregory Lee, Suefay Liu
Jan 30, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jun WeiZu-chuan Zhang
Mar 26, 2019·ACS Infectious Diseases·Wei ZhuXiaoyong Zhang

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