In vitro oligodendrogliotrophic properties of cell adhesion molecules in the immunoglobulin superfamily: myelin-associated glycoprotein and N-CAM

Journal of Neuroscience Research
A L GardM Schachner

Abstract

To determine if cell recognition molecules interact trophically with oligodendrocytes (OCs), their effect as growth substrates for differentiating oligodendroblasts was studied in primary culture. Oligodendroblasts purified from postnatal rat cerebrum by immunopanning were plated on substratum-bound cell adhesion molecules or extracellular matrix glycoproteins in chemically defined medium in which OCs terminally differentiate but survive poorly. Growth on myelin-associated glycoprotein (MAG) and neural cell adhesion molecule (N-CAM) selectively increased the number of viable cells per culture 2 weeks after plating as much as tenfold and sixfold, respectively, over background survival on an albumin substrate, whereas L1, tenascin-R, tenascin-C, fibronectin, and laminin were ineffective. Neither MAG nor N-CAM stimulated bromodeoxyuridine incorporation into cultures, indicating that enhanced proliferation did not contribute to better survival. Compared to growth on polyornithine alone, oligodendroblast differentiation in the added presence of MAG or N-CAM was qualitatively unchanged; > 90% of surviving cells developed into OCs that matured further by immunocytochemical and morphological criteria. A striking difference, however, wa...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N H SternbergerH D Webster
Jan 1, 1990·Annals of the New York Academy of Sciences·B D Trapp
Aug 22, 1985·Nature·M NobleU Rutishauser
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M GrumetG M Edelman
Feb 1, 1986·The Journal of Cell Biology·M Dubois-DalcqR A Lazzarini
Mar 1, 1986·The Journal of Cell Biology·C Ffrench-ConstantM C Raff
Oct 1, 1989·The Journal of Cell Biology·P PeshevaM Schachner
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R Bansal, S E Pfeiffer
Jan 1, 1988·Glia·J A Black, S G Waxman
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C Lagenaur, V Lemmon
Mar 1, 1995·The European Journal of Neuroscience·M FruttigerR Martini

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Citations

Nov 25, 2003·The American Journal of Pathology·Jamshid KhoshnoodiKarl Tryggvason
Jan 24, 2007·Journal of Neurochemistry·Richard H Quarles
Feb 18, 1999·Journal of Neurobiology·S ChenM Schachner
Jan 13, 2001·Brain Research. Developmental Brain Research·C A Ingraham, L J Rising
May 20, 2004·Experimental Cell Research·Pamela E Knapp, Mary H Adams

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