In vitro organ culture of the bovine intervertebral disc: effects of vertebral endplate and potential for mechanobiology studies

Spine
Cynthia R LeeM Alini

Abstract

Whole bovine coccygeal discs were cultured under static load, with or without vertebral endplates (VEPs), and assessed for cell viability, biochemical stability, biosynthetic activity, and biosynthetic responsiveness to changes in mechanical load. To assess the effects of VEPs on biochemical and cellular stability of disc cells during in vitro culture of large disc explants. To determine whether cultured discs could respond to mechanical perturbation. Previous methods for culturing the intervertebral disc have focused on rabbit and rat discs, but the small size of these discs limits the relevance of these culture systems to the human condition. Bovine coccygeal discs have similar dimensions to the human lumbar disc (i.e., similar size and nominal stresses), but long-term culture of these discs has not been reported. Bovine coccygeal discs were harvested with or without VEPs, cultured under static load (5 kg, approximately 0.25 MPa, in situ swelling pressure) for up to 1 week, and evaluated for changes in hydration, glycosaminoglycan content, cell viability, and biosynthetic activity. Additionally, the biochemical and biosynthetic response of discs cultured without VEP to increasing the load to a 20-kg (approximately 1 MPa, the ...Continue Reading

References

Jan 1, 1989·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R L SahJ D Sandy
Jan 1, 1981·Connective Tissue Research·S HolmA Nachemson
Jan 1, 1981·Connective Tissue Research·J P Urban, A Maroudas
Jan 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H OhshimaD H Bergel
May 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H OshimaH Tsuji
Jun 23, 2000·Archives of Biochemistry and Biophysics·L J BonassarS B Trippel
Oct 19, 2001·Clinical Orthopaedics and Related Research·K W LiR L Sah
Nov 21, 2001·Clinical Biomechanics·W C HuttonM Kozlowski
May 17, 2002·Annals of Medicine·Leena Ala-Kokko
Jun 8, 2002·Biochemical and Biophysical Research Communications·Jun ChenLori A Setton
Jul 29, 2003·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Sajjad RazaqJill P G Urban
Nov 1, 2003·The Spine Journal : Official Journal of the North American Spine Society·C CourtJ C Lotz
Jan 7, 2004·Journal of Biomechanics·Stephen J FergusonLutz P Nolte
Feb 6, 2004·Journal of Biomechanics·Andrew J L Walsh, Jeffrey C Lotz
Mar 5, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Jun ChenLori A Setton
Oct 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jeffery J MacleanJames C Iatridis
Nov 27, 2004·Spine·Howard S AnJames N Weinstein
Nov 27, 2004·Spine·Michele C BattiéEric Parent
Nov 27, 2004·Spine·Jill P G UrbanJeremy C T Fairbank
Dec 21, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Joseph J Sarver, Dawn M Elliott

❮ Previous
Next ❯

Citations

Jul 17, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Casey L KoreckiJames C Iatridis
Jul 17, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Mauro AliniHans Joachim Wilke
Feb 22, 2011·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Bernice JimLisbet Haglund
May 5, 2011·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Samantha C W ChanBenjamin Gantenbein-Ritter
Jun 25, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Cornelia Neidlinger-WilkeHans-Joachim Wilke
Jun 18, 2010·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Mao XieJianguo Zhou
May 24, 2007·Tissue Engineering·Damien M O'Halloran, Abhay S Pandit
Jun 15, 2011·Tissue Engineering. Part C, Methods·Lisbet HaglundThomas Steffen
Jun 4, 2008·Spine·Casey L KoreckiJames C Iatridis
Jan 22, 2009·Journal of Biomechanical Engineering·James C IatridisJeffrey P Laible
Feb 27, 2008·BMC Musculoskeletal Disorders·Sally RobertsJill P G Urban
Jun 4, 2008·Journal of Neurosurgery. Spine·Daniel HaschtmannJivko V Stoyanov
Feb 15, 2012·Journal of Visualized Experiments : JoVE·Samantha C W Chan, Benjamin Gantenbein-Ritter
Mar 30, 2013·The Spine Journal : Official Journal of the North American Spine Society·Li JinXudong Li
Sep 25, 2012·Acta Biomaterialia·Lucia K Y ChanKenneth M C Cheung
Nov 14, 2015·Tissue Engineering. Part C, Methods·Graciosa Q TeixeiraCornelia Neidlinger-Wilke
Feb 8, 2011·The Spine Journal : Official Journal of the North American Spine Society·Dong WangJames D Kang
Jul 6, 2010·The Spine Journal : Official Journal of the North American Spine Society·Hwan Tak HeeHee Kit Wong
Apr 21, 2007·Medical Engineering & Physics·Franck AccadbledPascal Swider
May 16, 2009·Journal of Tissue Engineering and Regenerative Medicine·Christine L Le MaitreJudith A Hoyland
Aug 19, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Daniel HaschtmannStephen J Ferguson
May 24, 2011·Journal of Cellular Physiology·Eleni Mavrogonatou, Dimitris Kletsas
Mar 5, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Dongrim SeolJames A Martin
May 10, 2011·Osteoarthritis and Cartilage·B A WalterJ C Iatridis
Apr 2, 2015·Osteoarthritis and Cartilage·P-P A VergroesenT H Smit
Nov 22, 2011·Journal of Biomechanics·Robert A HartmanGwendolyn A Sowa
Dec 30, 2014·The Spine Journal : Official Journal of the North American Spine Society·Takuto KurakawaKotaro Nishida
Nov 18, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Pei LiQiang Zhou
Jun 9, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Adam C AbrahamSimon Y Tang
Feb 23, 2010·The Spine Journal : Official Journal of the North American Spine Society·Samantha C W ChanKeita Ito

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.