In Vitro Osteogenesis Stimulation via Nano-Hydroxyapatite/Carbon Nanotube Thin Films on Biomedical Stainless Steel

Materials
Natalia M MartinelliF R Marciano

Abstract

We evaluated the electrophoretic deposition of nanohydroxyapatite/superhydrop hilic multiwalled carbon nanotube composites (nHAp/MWCNT) onto stainless steel biomedical alloys for applications in bone tissue engineering. First, nHAp/MWCNT composites were dispersed into 0.042 mol·L-1 of Ca(NO₃)₂·4H₂O + 0.025 mol·L-1 NH₄H₂PO₄ electrolytes (pH = 4.8) at two different concentrations. Next, a voltage of -2 V was applied using 316L stainless steel as a working electrode (0.27 cm²), a high-purity platinum coil wire was used as the auxiliary electrode, and an Ag/AgCl (3 M) electrode was used as the reference electrode. The nHAp/MWCNT composites were characterized by transmission electron microscopy. The deposited nHAp and nHAp/MWCNT films were characterized by profilometry, scanning electron microscopy, X-ray diffractometry and Raman spectroscopy. Human osteoblast cells were cultivated with the different materials and in vitro cytotoxicity was evaluated using lactate dehydrogenase (LDH) assay. The osteogenesis process was evaluated by mRNA levels of the three genes that are directly related to bone repair: Alkaline Phosphatase, Osteopontin and Osteocalcin. We showed that rough, crystalline apatite thin films containing phases of nHAp we...Continue Reading

Citations

Jun 30, 2019·Journal of Materials Science. Materials in Medicine·Luana Marotta Reis de VasconcellosAnderson Oliveira Lobo

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Methods Mentioned

BETA
chemical modification
transmission electron microscopy
X-ray
electrophoresis
PCR
thermal treatment

Software Mentioned

GraphPad Prism InStat
IDT
Blast
Primer
GraphPad Prisma

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