In vitro osteogenic potential of an experimental calcium silicate-based root canal sealer

Journal of Endodontics
Thomas E BryanFranklin R Tay

Abstract

This in vitro study compared the cytotoxicity and osteogenic potential of an experimental calcium silicate-based sealer with an epoxy resin-based sealer (AH Plus; Dentsply Caulk, Milford, DE) and a zinc oxide-eugenol-based sealer (Pulp Canal Sealer; SybronEndo, Orange, CA). Disks prepared from the respective sealer and from Teflon (negative control) were placed in direct contact with a MC3T3-E1 osteogenic cell line at 6 weekly intervals after immersion in a culture medium. Succinic dehydrogenase activities were evaluated using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Extracts from these sealers after the 6-week immersion period were investigated also by MTT assay. Aged sealers were then switched to an osteogenic medium for examination of the alkaline phosphatase activity and mineralization of extracellular matrices produced by the differentiated cells. All sealers exhibited severe toxicity after 24 hours, after which toxicity decreased gradually over the experimental period except for Pulp Canal Sealer, which remained severely toxic. Toxicity of the extracts derived from the sealers was concentration dependent, with those derived from the experimental sealer being the least cytotoxic at a 1:10...Continue Reading

Citations

May 21, 2013·Journal of Endodontics·Cláudia RodriguesMaria Helena Fernandes
Sep 14, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Sasha Dimitrova-NakovMichel Goldberg
Oct 24, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Eduardo A BortoluzziFranklin R Tay
Sep 24, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Martha Goël BrackettJohn C Wataha
Feb 4, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Martha Goël BrackettJohn C Wataha
Nov 30, 2013·Journal of Dentistry·Raqueli ViapianaJ Camilleri
Jun 1, 2016·International Journal of Biomaterials·Afaf Al-Haddad, Zeti A Che Ab Aziz
Apr 19, 2011·Journal of Endodontics·Bethany A LoushineFranklin R Tay
Jul 22, 2017·European Journal of Dentistry·Sandra ChakarIssam Khalil
Apr 23, 2013·Clinical Oral Investigations·Cláudia RodriguesMaria H Fernandes
Dec 5, 2012·Dental Materials Journal·Zeliha YilmazAhmet Serper
Dec 11, 2019·Materials·Diogo Afonso FonsecaManuel Marques Ferreira
Sep 12, 2019·Brazilian Oral Research·Francine BenettiLuciano Tavares Angelo Cintra
Apr 25, 2021·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Min-Gyu ParkHyeon-Cheol Kim

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