Abstract
In vitro pharmacodynamic properties of colistin methanesulfonate and amikacin were investigated by studying time-kill kinetics and post-antibiotic effect (PAE) against strains of Pseudomonas aeruginosa isolated from patients with cystic fibrosis. Synergy was investigated at 0.5 ×, 1 × and 5 × MIC of antibiotics using time-kill curve method. PAEs were determined by the standard viable counting method where bacteria in the logarithmic phase of growth were exposed for 1 h to the antibiotics at 1 × or 20 × MIC, alone and in combinations. Synergy and additive effects were detected at 1 × MIC, at 24 h. Some of the strains produced an earlier synergistic effect at 12 h. No antagonism was observed. Colistin methanesulfonate and amikacin produced PAEs 1.16 ± 0.10 to 2.25 ± 0.16 h and 0.96 ± 0.15 to 2.69 ± 0.32 h, respectively. When the antibiotics were used in combination the PAEs were prolonged to a value of 3.88 ± 0.25 h. Consequently, the Findings of this study may play useful role in selecting the appropriate combinations when a single agent is inadequate, and may have important information for optimizing the dose intervals.
References
Nov 1, 1995·Chemotherapy·A A Gerçeker, G Otük
Aug 1, 1997·Antimicrobial Agents and Chemotherapy·D A SteinbergJ C Fiddes
Feb 27, 1999·Antimicrobial Agents and Chemotherapy·A D KashubaJ S Bertino
Jul 2, 1999·Antimicrobial Agents and Chemotherapy·M J RybakG L Drusano
Dec 6, 2000·The European Respiratory Journal·G DöringD J Touw
Feb 22, 2001·Antimicrobial Agents and Chemotherapy·J LiK Coulthard
Feb 22, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Caroline RynnDavid S. Reeves
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
Sep 6, 2003·Journal of Clinical Microbiology·Melissa B Miller, Peter H Gilligan
Dec 29, 2004·International Journal of Antimicrobial Agents·Jian LiKingsley Coulthard
Dec 27, 2005·Antimicrobial Agents and Chemotherapy·Valerie AloushYehuda Carmeli
Mar 2, 2006·Critical Care : the Official Journal of the Critical Care Forum·Matthew E Falagas, Sofia K Kasiakou
Oct 31, 2007·Antimicrobial Agents and Chemotherapy·Glenn A PankuchPeter C Appelbaum
Mar 7, 2008·Chemotherapy·Cağla Bozkurt Güzel, A Alev Gerçeker
May 10, 2008·Journal of Chemotherapy·G TaccettiF Festini
Dec 10, 2009·Chemotherapy·Berna Ozbek, Gulten Otuk
Jan 12, 2010·Indian Journal of Medical Microbiology·B Ozbek, G Otuk
Citations
Feb 11, 2014·Drugs·Daniel Conole, Gillian M Keating
Mar 19, 2014·The Journal of Infection·Nihal MartisVéronique Blanc
Oct 22, 2015·Expert Review of Anti-infective Therapy·Yiying CaiAndrea L Kwa
Mar 24, 2017·The Journal of Antimicrobial Chemotherapy·Yu-Feng ZhouJian Sun
Feb 26, 2021·Pharmacological Reviews·Sue C NangJian Li