PMID: 9427520Jan 14, 1998Paper

In vitro phosphorylation of acetylcholinesterase at non-consensus protein kinase A sites enhances the rate of acetylcholine hydrolysis

Brain Research. Molecular Brain Research
M GrifmanHermona Soreq

Abstract

Here, we report that the catalytic subunit of cAMP-dependent protein kinase (PKA) but not casein kinase II or protein kinase C phosphorylates recombinant human acetylcholinesterase (AChE) in vitro. This enhances acetylthiocholine hydrolysis up to 10-fold as compared to untreated AChE, while leaving unaffected the enzyme's affinity for this substrate and for various active and peripheral site inhibitors. Alkaline phosphatase treatment enhanced the electrophoretic migration, under denaturing conditions, of part of the AChE proteins isolated from various mammalian sources and raised the isoelectric point of some of the treated AChE molecules, indicating that part of the AChE molecules are also phosphorylated in vivo. Enhancement of acetylthiocholine hydrolysis also occurred with Torpedo AChE, which has no consensus motif for PKA phosphorylation. Further, mutating the single PKA site in human AChE (threonine-249) did not prevent this enhancement, suggesting that in both cases it was due to phosphorylation at non-consensus sites. In vivo suppression of the acetylcholine hydrolyzing activity of AChE and consequent impairment in cholinergic neurotransmission occur under exposure to both natural and pharmacological compounds, including...Continue Reading

References

Feb 28, 1991·The New England Journal of Medicine·J M Astrachan
Aug 5, 1987·Journal of Molecular Biology·S MillerC Chothia
May 1, 1985·Journal of Neurology, Neurosurgery, and Psychiatry·E K PerryR H Perry
Jan 1, 1995·Progress in Histochemistry and Cytochemistry·P G Layer, E Willbold
May 1, 1993·Trends in Neurosciences·C Léna, J P Changeux
Jun 8, 1995·Nature·V A PieriboneP Greengard
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·C PatriotisP N Tsichlis
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·D I KreimerL Weiner
Apr 6, 1994·JAMA : the Journal of the American Medical Association·M J KnappS I Gracon
Nov 1, 1993·Journal of Neurochemistry·B M Schmidt, R J Rylett
Jul 1, 1993·Progress in Neurobiology·J MassouliéF M Vallette
Feb 1, 1993·Cellular and Molecular Neurobiology·M FischerM Gorecki
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R Ben Aziz-AloyaH Soreq
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D R RipollJ L Sussman
Feb 2, 1996·The Journal of Biological Chemistry·K IchtchenkoT C Südhof

❮ Previous
Next ❯

Citations

Jul 10, 2004·Pflügers Archiv : European journal of physiology·Craig B NeylonPeter H Reinhart
Feb 14, 2012·Molecular Neurobiology·Giselli ScainiEmilio L Streck
Feb 9, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Rosane Souza da SilvaCarla Denise Bonan
Jun 11, 2008·Chemico-biological Interactions·Jean MassouliéSuzanne Bon
Mar 27, 2016·Science Signaling·Paulo Sérgio L de OliveiraDeborah Schechtman
Oct 11, 2012·Neurochemistry International·Gabriela K FerreiraEmilio L Streck
Jan 4, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Milena JankowskaMaria Stankiewicz
Feb 28, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alain Robichon
Sep 11, 2004·Assay and Drug Development Technologies·Can C OzbalColin J H Brenan
Oct 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Milena JankowskaMaria Stankiewicz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.