In vitro placental model optimization for nanoparticle transport studies.

International Journal of Nanomedicine
L CartwrightErik Rytting

Abstract

Advances in biomedical nanotechnology raise hopes in patient populations but may also raise questions regarding biodistribution and biocompatibility, especially during pregnancy. Special consideration must be given to the placenta as a biological barrier because a pregnant woman's exposure to nanoparticles could have significant effects on the fetus developing in the womb. Therefore, the purpose of this study is to optimize an in vitro model for characterizing the transport of nanoparticles across human placental trophoblast cells. The growth of BeWo (clone b30) human placental choriocarcinoma cells for nanoparticle transport studies was characterized in terms of optimized Transwell(®) insert type and pore size, the investigation of barrier properties by transmission electron microscopy, tight junction staining, transepithelial electrical resistance, and fluorescein sodium transport. Following the determination of nontoxic concentrations of fluorescent polystyrene nanoparticles, the cellular uptake and transport of 50 nm and 100 nm diameter particles was measured using the in vitro BeWo cell model. Particle size measurements, fluorescence readings, and confocal microscopy indicated both cellular uptake of the fluorescent polyst...Continue Reading

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Citations

Jun 9, 2012·International Journal of Nanomedicine·Ye WangYi-Ping Hu
Nov 4, 2015·Placenta·Jian-Pei HuangChie-Pein Chen
Mar 18, 2016·Journal of Photochemistry and Photobiology. B, Biology·Muthuraman PanduranganDoo Hwan Kim
Mar 22, 2014·Basic & Clinical Pharmacology & Toxicology·Line MathiesenLisbeth E Knudsen
May 16, 2015·Archives of Toxicology·Hedwig M BraakhuisHans Bouwmeester
Jul 7, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Samantha K KloetIvonne M C M Rietjens
Jun 9, 2015·Reproductive Toxicology·Karin Sørig HougaardFlemming R Cassee
Mar 25, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Irina BurdRangaramanujam M Kannan
Jan 25, 2017·ACS Applied Materials & Interfaces·Feiyang DengQiang Zhang
Mar 31, 2018·Scientific Reports·Leonie AengenheisterTina Buerki-Thurnherr

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Methods Mentioned

BETA
transmission electron microscopy
dynamic light scattering

Software Mentioned

Imaris Bitplane
Excel
Leica confocal

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