In vitro potential anthelmintic activity of Biophytum petersianum on Haemonchus contortus

Veterinary World
Priyo SambodoSudarmanto Indarjulianto

Abstract

Haemonchus contortus is a major problem in small ruminants in Indonesia. The frequent use of the anthelmintic drugs has given rise to drug-resistant populations which increase the need for new anthelminthic compounds, particularly from endemic plants. This study evaluated the in vitro effects of Biophytum petersianum crude aqueous extract (BAE) as an anthelmintic compound against H. contortus adult worm isolated from goats. Adult worm collected from naturally infected abomasums were obtained from slaughtered goats on the day of slaughter. BAE was prepared in six different concentrations (10, 20, 40, 60, 80, and 100 mg/ml) which were tested for their efficacies on ten actively moving worms. Ivermectin (1 mg/ml) was included as a reference drug, while saline water was included as a control. The dead worms from anthelmintic test then went through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscopy (SEM). Highest mean mortality in treatments group both at 2 h and 4 h observations was BAE 10%. The SDS-PAGE analysis revealed the presence of five protein bands with molecular weights 9.3, 17.1, 50.0, 63.2, and 72.7 kDa based on BAE 10%. The SEM changes observed in the in vitro trials re...Continue Reading

References

Jul 1, 1991·Reviews of Infectious Diseases·S C ChenT C Sorrell
Apr 25, 2006·Trends in Parasitology·Hervé HosteSimone O Hoskin
Dec 19, 2012·Experimental Parasitology·C Martínez-Ortíz-de-MontellanoH Hoste
May 18, 2016·Veterinary World·Norisal Binti NasaiMohammed Ariff Bin Omar
Sep 9, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Amit Kumar JaiswalDaya Shanker

❮ Previous
Next ❯

Citations

Jan 8, 2021·Journal of Pharmacopuncture·Innocent SutngaAnanta Choudhury
Sep 4, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Gerardo Jiménez-PenagoPedro Mendoza-de Gives

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
motility assay

Software Mentioned

SPSS

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

© 2022 Meta ULC. All rights reserved